Easy and Quick DNA Purification

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England BioLabs

Product Name:

Monarch Genomic DNA purification kit

Catalog Number:

T3010L

Clone Number:

N/A

Easy and quick purification of genomic DNA from various samples (cultured cells, whole blood and animal tissue). Allow the obtention of good quality DNA, without protein nor solvent contamination.

Experimental Design and Results Summary

Applications

Molecular Biology

Sample

Mammalian cells

Primary Incubation

Cell lysis with proteinase K, RNase and lysis buffer for 5 min at 56°C

Blocking Agent

None

Secondary Incubation

gDNA binding buffer

Tertiary Incubation

gDNA Wash Buffer

Detection

Nanodrop

Results Summary

This kit allow the purification of intact genomic DNA from CRISPR genetically modified cells. I then use this DNA directly for sequencing, in order to determine if the CRISPR mutation has been integrated.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Obtention of good quality DNA.

The Bad

Expensive

The Bottom Line

Obtention of intact DNA with good purity.

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