Abcam
Anti-Vinculin antibody
ab91459
N/A
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I used the anti-vinculin antibody at a dilution of 1:2000 as a loading control for western blot. I used 20 ug protein/lane of mouse brain homogenate and consistently get bands around ~120 kDa.
Western Blot
Mouse brain homogenate
Overnight at 4C
3% BSA in TBS-T
1 hour at room temp
Luminol
The vinculin antibody is very reliable, and I consistently get good signal when using it as a control for normalization when doing western blots.
Reliable and good signal.
Antibody very reliable, highly recommend.