Millipore Sigma
Anti-MAFB antibody
HPA005653
Rabbit polyclonal
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MAFB is an important transcription factor for different cell type. In the kidney it is crucial for the differentiation and adult homeostasis of the podocytes. This antibody allowed us to study the role of this transcription factor in those processes.
ChIP
Dissociated embryonic kidneys
1ug antibody for 5ug sonicated chormatin, ON at 4°C rocking
None
Protein G coated beads, 30ul/ug of antibody, 2 hours at 4°C rocking
qPCR on precipitated chromatin or sequencing
This antibody gave us good results when tested by qPCR on precipitated chromatin. We moved on with the whole genome sequencing and we found that the signal to noise ratio was not as good as expected, but this might be intrinsic of the transcription factor properties.
N/A
Validated for ChIP
Recommended antibody for MAFB chromatin immuno-precipitation. The "Prestige" label of this product is well deserved for this type of analysis (a bit less for other applications such as IF or IHC).