Single Mix to Insert Small Mutations into Plasmids

October 27, 2022

BlueskyReddit
University of Cote d'Azur (UCA)
LP2M
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

KLD Enzyme Mix

Catalog Number:

M0554S

Small plasmid modifications (point mutations, insertion/deletions..) requires serial steps to obtain the final construct. This kit allows you to reduce all these steps to a single incubation.

Experimental Design and Results Summary

Application

Cloning

Starting Material

Plasmids

Tips

Some optimization of the incubation time is required.

Results Summary

This product reduces the step required to introduce small modifications into plasmid. We faced some efficiency issues that we partially solved increasing the incubation time.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Single incubation protocol.

The Bad

Low efficiency

The Bottom Line

This kit is very helpful to reduce the hands on time when trying to modify a plasmids. It also avoids to re-synthesise/cloning the entire construct. In the other hand, we observe very low efficiency when following the proposed protocol. Longer incubation and plasmid clean-up after PCR amplification partially solve this issue.

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