KLD Enzyme Mix M0554S from New England Biolabs

Product Specs
ItemKLD Enzyme Mix
CompanyNew England Biolabs
Price $340.00 Inquire
Catalog NumberM0554S
Size25 reactions
ApplicationsDNA Manipulation
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Divergent Rickettsia species exhibit distinct mechanisms of actin-based motilityJournal of Cell BiologyJuly 6, 2026Meghan C. Bacher, Julie E. Choe, Jiawen Jiang, Joanna M. Idrovo, Joshua T. Del Mundo, Michal Hammel, Matthew D. Welch5’-TCCCCCACCGCTTCCACCGCCTTCATCACCACCAGTAATC-3’). The resulting PCR products where ligated using the KLD reaction mix (New England Biolabs, M0554S). To generate plasmids pGEX-GST-Sca2/6–44 (aa 44–175) and pGEX-GST-Sca2/6–357 (and aa 357–464) for anti-Sca2/6

(2) Evolutionarily conserved short linear motifs drive actin filament bindingNat Cell BiolJuly 6, 2026Themistoklis Paraschiakos, Biao Yuan, Michael Hecht-Bucher, Kostiantyn Sopelniak, Pasquale Cervero, Lisa Simon, Ksenija Zonjic, Dominic Eggers, Franziska Selle, Jing Li, et al.Agilent Technologies, Inc.). Alternatively, the PCR products were processed using a kinase–ligase–DpnI enzyme mix (M0554S, New England Biolabs Inc.) to remove the template and ligate the new amplicon. For bacterial expression,

(3) Bacterial lipid synthesizing enzymes PlsY and PlsC utilize both stereo-forms of glycerol-phosphateEMBO ReportsJune 12, 2026Philipp Rieche, Mirthe Hoekzema, Sergiy Gan, Greetje A Berrelkamp-Lahpor, Adanna W Ezissi, Marten Exterkate, Arnold J M DriessenEcoRI",New England Biolabs,"Cat: R3193S, R3156S, R3136S, R3101S" KLD Enzyme Mix,New England Biolabs,Cat: M0554S Gibson Assembly Mix,New England Biolabs,Cat: E2611L Ampicillin,Sigma,Cat: A9518 Kanamycin,Roth,Cat:

(4) Atg23 interacts with both the N- and C-termini of Atg9 via a hydrophobic binding pocketJournal of Biological ChemistryJune 12, 2026Zhanibek Bekkhozhin, Kelsie A. Leary, Michael J. Ragusaampicillin (Fisher BP1760-25), kanamycin (Gibco 11815-032), Q5 2X master mix (M0492S), KLD enzyme mix (NEB M0554S), 2× Gibson Assembly master mix (NEB E2611S), T4 ligase (NEB M0202S); BamHI-HF, SalI-HF, NcoI-HF, NotI-HF,

(5) Mechanistic design of cell-penetrating disruptors for phospho-dependent TACC3-CHC interactionStructure (London, England : 1993)June 4, 2026Gunning V, Batchelor M, Alexander KK, Walko M, Dill TC, Burgess SG, Royle SJ, Kennedy EJ, Bayliss R.CY-SC201 Critical commercial assays,Critical commercial assays,Critical commercial assays KLD Enzyme Mix,NEB,Cat# M0554S Q5 Mutagenesis kit,NEB,Cat# E0554S Pacific Blue Annexin V apoptosis detection kit,BioLegend,Cat# 640928 Deposited

(6) TraN variants mediate conjugation species specificity of IncA/C, IncH, and Acinetobacter baumannii plasmidsJournal of BacteriologyApril 23, 2026Shan He, Sophia David, Jaie Rattle, Julia Sanchez-Garrido, Wen Wen Low, Joshua L. C. Wong, Konstantinos Beis, Gad Frankelvectors was performed according to the Q5 Site-Directed Mutagenesis Kit protocol (New England Biolabs, M0554S). Primers used to generate the mutagenesis vectors and for screening are listed in Table S4. All mutations

(7) Force-modulated structural landscape of the catch bonding F-actin crosslinker α-actinin-4bioRxivApril 16, 2026Alfred C. Chin, Fatemah Mukadum, Matthew J. Reynolds, Glen M. Hocky, Gregory M. AlushinGibson assembly (New England Biolabs, E2611L) or kinase-ligase-DpnI (KLD) cloning (New England Biolabs, M0554S). All plasmids were cloned and propagated in NEB 5-alpha competent E. coli cells (New England Biolabs,

(8) Protocol for MR1-ligand cross-linking to discover metabolite antigens by proteomicsSTAR ProtocMarch 20, 2026Hannah Thomas, Garry Dolton, Lucia F. Cardo, Nicola Ternette, Andrew K. Sewell, Thierry Schmidlinassays ,, Prime star PCR Master Mix,Takara,Cat# R053A Kinase-Ligase-Dpn1 enzyme mix,New England Biolabs,Cat# M0554S HiPure Miniprep kit,ThermoFisher Scientific,Cat# K210002 MycoAlert™ kit,Lonza,Cat# LT07-318 LIVE/DEAD®

(9) The yeast phosphofructokinase β-subunit has RNA unwinding activity and modulates cell cycle progressionNucleic Acids ResMarch 12, 2026Waleed S Albihlal, Ana M Matia-González, Tobias Schmidt, Wieland Mayer, Joe Bryant, Martin Bushell, Dierk Niessing, Matteo Barberis, Alexander Schmidt, Jürgen J Heinisch, et al.polymerase chain reaction (PCR) Purification Kit (Qiagen, 28104), Kinase-Ligase-DpnI (KLD) enzyme mix (New England Biolabs, M0554S), LR Clonase II plus kit (Thermo Fisher, 12538120), Q5 site-directed Mutagenesis kit (NEB, E0554S),

(10) Human LFA-1 governs T cell immune surveillance of the skinScience ImmunologyFebruary 27, 2026Ahmad Yatim, Leila Youssefian, Aida Idani, Haralambos Mourelatos, Nasrin Alipour Alipour Olyaei, Laleh Habibi, Jessica N. Peel, Maira Gaballa, Colin Kim, Danyel Lee, et al.treated with an enzyme mixture containing kinase, ligase, and DpnI (New England Biolabs, catalog no. M0554S) for 5 min at room temperature to facilitate plasmid circularization and to degrade the parental plasmid.

(11) Mechanistic insights into RNA chaperoning by Ro60 and La autoantigensCellFebruary 19, 2026Hyeyeon Nam, Justin C. Deme, Soyeong Sim, Marco Boccitto, Susan M. Lea, Sandra L. WolinC-terminus. For La 222–336, aa 337–408 were deleted from La 222–408 using KLD enzyme mix (New England Biolabs M0554S) and oligonucleotides “La336_5p” and “La336_3p” according to the manufacturer’s instructions. The proteins

(12) GRK phosphorylation drives β-arrestin–independent internalization of chemokine receptor CXCR5Journal of Biological ChemistryFebruary 1, 2026Joseph M. Crecelius, Ya Zhuo, Aaren R. Manz, Julia Drube, Stefan Schulz, Carsten Hoffmann, Adriano Marchesesubstitution for Lys-220 and then circularized using the Kinase, Ligase, and Dpn1 (KLD) enzyme mix (NEB, Cat# M0554S) according to manufacturer’s instruction. The FLAG-tagged CXCR5-NLuc plasmid was made by PCR amplification

(13) Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding PocketbioRxivDecember 22, 2025Zhanibek Bekkhozhin, Kelsie A Leary, Michael J Ragusaampicillin (Fisher BP1760-25), kanamycin (Gibco 11815-032), Q5 2X master mix (M0492S), KLD enzyme mix (NEB M0554S), 2x Gibson Assembly master mix (NEB E2611S), T4 ligase (NEB M0202S); BamHI-HF, SalI-HF, NcoI-HF, NotI-HF,

(14) Low-order assemblies drive oncogenic RTK fusion signaling without condensationbioRxivDecember 13, 2025David Gonzalez-Martinez, Thomas R. Mumford, Delaney Wilde, Sofia Wissert, Yuzhi (Carol) Gao, Emily Brackhahn, Richard Kriwacki, Elizabeth Rhoades, Lukasz J. Bugaj(New England Biolabs (NEB), #E2621); blunt end ligation using NEB T4 ligase (NEB, #M0202) or NEB KLD (NEB, #M0554S); or Golden Gate cloning using BbsI (NEB, R3539S). EML4-ALK variants were cloned downstream of (C-terminal

(15) Assessing the function of the alternative electron transport chain in the Cryptosporidium parvum mitosomemBioDecember 10, 2025Silu Deng, Wandy Beatty, L. David Sibleyusing primers to edit the sequence followed by treatment with KLD enzyme kit (New England Biolabs, #M0554S). To generate repairing templates for gene deletions, homology repair fragments flanking the mCherry-Nluc-P2A-NeoR

(16) Discovery of an allosteric binding site for anthraquinones at the human P2X4 receptorNat CommunDecember 4, 2025Jessica Nagel, Vigneshwaran Namasivayam, Stephanie Weinhausen, Juan Sierra-Marquez, Younis Baqi, Hashem Ali M. Al Musawi, Aliaa Abdelrahman, Victoria J. Vaaßen, Jonathan G. Schlegel, Lisa Taplick, et al.manufacturer’s instructions. Point mutations were introduced by site-directed mutagenesis using KLD Enzyme Mix (M0554S, New England Biolabs, USA). All constructs were verified by full-length cDNA sequencing (Eurofins Genomics,

(17) Toxoplasma effector TgROP1 establishes membrane contact sites with the endoplasmic reticulum during infectionNature MicrobiologyNovember 25, 2025Chahat Mehra, Jesús Alvarado Valverde, Ana Margarida Nogueira Matias, Francesca Torelli, Tânia Catarina Medeiros, Julian Straub, James D. Asaki, Peter J. Bradley, Katja Luck, Steffen Lawo, et al.pMXs–myc–GFP1-10 plasmid28. The PCR product was treated with the Kinase, Ligase, and DpnI enzyme mix (NEB M0554S) according to manufacturer’s protocol. To create ERM-targeted GFP1-10, the Sac1 ER targeting sequence

(18) Engineered Leaf‐Branch Compost Cutinase with Enhanced PET Degradation Across a Broad Temperature RangeChemSusChemNovember 19, 2025Kavita G. Ramnath, Colin T. Pierce, Guillem Casadevall, Silvia Osuna, Jadyn Ide‐Pech, Bryan Zeng, Vishnu Iyer, Romas J. Kazlauskas, Richard A. Grossa final extension step of 72°C for 2 min. The PCR products were treated with a KLD enzyme mix (NEB M0554S), which phosphorylates the 5′ ends of the linearized PCR products, ligates the phosphorylated ends,

(19) MMP9 shapes cell mechanics to enable collective invasion in cancerNPJ Syst Biol ApplOctober 29, 2025Asadullah, Sarbajeet Dutta, Sumon Kumar Saha, Anisha Karmakar, Nikita Sharma, Sudikshaa Vijayakumar, Shamik SenSubsequently, the PCR product was used in Kinase-Ligase-DpnI (KLD) reaction using KLD Mixture (NEB, Cat # M0554S) according to the manufacturer’s protocol. The clone was verified by DNA sequencing. Lentiviral particles

(20) The twist-and-squeeze activation of CARF-fused adenosine deaminase by cyclic oligoadenylatesThe EMBO JournalOctober 17, 2025Charlisa Whyms, Yu Zhao, Doreen Addo-Yobo, Huan He, Arthur Carl Whittington, Despoina Trasanidou, Carl Raymund P Salazar, Raymond H J Staals, Hong LiPrestained Protein Ladder,Thermo Fisher Scientific,26616 KLD Enzyme Mix 25 rxns,New England Biolabs,M0554S SYBR Gold Nucleic Acid Gel Stain,Invitrogen,S11494 Software,Software,Software Graphpad Prism,https://www.graphpad.com/,N/A CryoSPARC,https://cryosparc.com/, Phenix,https://phenix-online.org/, Chimera,https://www.rbvi.ucsf.edu/chimerax/, Relion,https://github.com/3dem/relion, Coot,https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/, Topaz,https://github.com/tbepler/topaz, Reagents

Write a Review

(1)

10/27/2022Single Mix to Insert Small Mutations into Plasmids Filippo MassaSmall plasmid modifications (point mutations, insertion/deletions..) requires serial steps to obtain the final construct. This kit allows you to reduce all these steps to a single incubation.
(1)

12/10/2019Efficient Site-Directed Mutagenesis Yashavantha VishweshwaraiahA very efficient enzyme mix that can save time and can greatly improve the site-directed mutagenesis results.

KLD Enzyme Mix

KLD Enzyme Mix from New England Biolabs

Description

New England Biolabs

Citations (105)

Reviews

KLD Enzyme Mix from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map