Thermo Fisher Scientific
alpha Tubulin Monoclonal Antibody (DM1A)
62204
N/A
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I used this tubulin antibody as a cytosolic marker for cellular fractionation experiments. This was a terrific antibody with no background and you get strong and clean signal.
Western Blot
K562, HCT116
1:10,000 either overnight at 4C or RT for 1-2 hrs
Milk
1:10,000 licor 800 goat anti mouse
LiCor
Helped show my cellular fractionation was working well. clean signal in cytosol fractions and little to no signal in nuclear fraction.
Easy to use
None
Would highly recommend this antibody as a cytosolic marker. Great clean signal.