Great Loading Control GAPDH Antibody

October 25, 2022

BlueskyReddit
CTB
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

GAPDH antibody

Catalog Number:

GTX627408

Clone Number:

[GT239]

Info:Supplier Page View Product Specs

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This GAPDH antibody from GenTex was a great, easy to use loading control and cytosol marker for western blot. High signal, low background. I would highly recommend.

Experimental Design and Results Summary

Applications

Western Blot

Sample

K562, HCT116

Primary Incubation

1:1,000

Blocking Agent

Milk

Secondary Incubation

1:10,000 licor 800 goat anti mouse

Tertiary Incubation

N/A

Detection

Licor

Results Summary

I used this antibody as a loading control to make sure all lanes of my western blots were loaded evenly. This was a great antibody helping me show my cytosolic fractions were very clean.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Great antibody with high signal, very clean, no extra bands.

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