Poor GAPDH Antibody

October 25, 2022

BlueskyReddit
CTB
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-GAPDH Antibody (6C5)

Catalog Number:

sc-32233

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: GAPDH Antibodies

Used for WB as loading control: tons of excessive banding. Do not recommend this as a control antibody.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HCT116

Primary Incubation

1:5000

Blocking Agent

Milk

Secondary Incubation

1:10,000 licor 800 goat anti mouse

Tertiary Incubation

N/A

Detection

Licor

Results Summary

Trying to use as a cytosolic loading control but it did not look good. Excessive banding all throughout. Unexpected for just a simple loading control.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Not much

The Bad

Poor antibody.

The Bottom Line

Do not recommend.

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