Good Phospho-STAT3 Antibody

Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Phospho-Stat3 (Tyr705) (D3A7)

Catalog Number:

#9145

Clone Number:

N/A

To determine whether IL-6 gene transfer affect phosphorylated STAT3 expression. The results showed IL-6 enhanced STAT3 protein phosphorylation in obese mice.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mice liver tissue

Primary Incubation

1:1000 overnight at 4 degree

Blocking Agent

5% milk

Secondary Incubation

1:3000 1 hour at room temperature

Tertiary Incubation

1 minute at room temperature

Detection

ECL

Results Summary

To determine whether IL-6 gene transfer affect phosphorylated STAT3 expression. The results showed IL-6 enhanced STAT3 protein phosphorylation in obese mice.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good antibody for western blot.

The Bad

N/A

The Bottom Line

Good phosphorylated antibody for WB.

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