Good GATA3 Antibody for IF

October 19, 2022

BlueskyReddit
Institute of Microbiology, Infectious Diseases and Immunology
Charité – Universitätsmedizin Berlin
Principal Investigator

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Quality of Results

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Company:

BD Biosciences

Product Name:

Alexa Fluor® 647 Mouse anti-GATA3

Catalog Number:

560078

Clone Number:

L50-823

Info:Supplier Page 

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As a positive control for Gata3 staining, we used the virgin mammary gland, where E-Cadherin+ luminal cells express Gata3.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed 20 μm cryosection of mouse virgin mammary gland

Primary Incubation

1:20 overnight at room temperature

Blocking Agent

1% BSA, 0.3% Triton X, 2% mouse serum, 2% rat serum

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Images were acquired using an inverted Zeiss LSM780 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Excellent nuclear staining of Gata3 (red) in E-Cadherin+ (green) luminal cells of virgin mammary gland.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Excellent staining of Gata3.

The Bad

None

The Bottom Line

Very nice nuclear staining of Gata3, with no/minimal background.

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