Reliable and Cost Effective CD8 Antibody

Immunology
UiO
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-human CD8 Antibody

Catalog Number:

344704

Clone Number:

SK1

Info:Supplier Page View Product Specs

Browse Similar Products: CD8 Antibodies

Our research is based on utilizing CD8+ T-cells for therapeutic purposes. CD8 antibodies are commonly used in our lab for our established cellular assays.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

1:200, 20 min RT

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

Using the FITC-aCD8 antibody, a clear separation of the CD8 T-cells is observed, when PBMCS were stained with it.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright and reliable staining.

The Bad

None

The Bottom Line

The antibody works well and is reliable between different batches. We use other fluorochrome versions of the antibody and the FITC one is similarly bright as the PE/APC versions. In addition, our titration experiments show that less volume is required to achieve similar results, thus making it more cost-effective.

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