Beckman Coulter
CD45-FITC, J33, 2 mL, ASR
IM0782U
J33
Info:Supplier Page
Browse Similar Products: CD45 Antibodies
CD45 staining is central to most WBC immunophenotyping assay. It stains all leukocytes to allow the separation of these cells from RBC and platelets. Using a good antibody is important. We opted for this one because it is a good clone.
Flow Cytometry
WBC (human)
15 minutes (RT)
1% FBS
None
FL-1 channel (using blue laser)
The results are as expected. Lymphocytes (red) stain brightly, monocytes (green) show intermediate expression, and granulocytes (blue) show dim CD45. They are all positive compared to the red cells and platelets (gray).
N/A
Easy to use. Cheap.
No bad issues to report.
This is a good antibody that will fit in with most platforms. It is relatively cheap, and quite easy to use.