Protein Carbonyl Content Assay Kit Works Ok, but Expensive

Yale University
Internal Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Protein Carbonyl Content Assay Kit

Catalog Number:

ab126287

We wanted to analyze the levels of protein carbonylation in plasma and platelets. Worked better for plasma as compared to platelets

Experimental Design and Results Summary

Application

Oxidative protein modifications- protein carbonylation

Starting Material

Human platelets and plasma samples

Tips

Begin with higher platelet protein concentrations.

Results Summary

This helped us get supportive evidence of oxidative protein modifications in presence of hydrogen peroxide in platelet and plasma samples.

DOI or PMID #

10.1161/CIRCRESAHA.121.320530

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Summary

The Good

Works great once optimized. pretty consistent with value ranges.

The Bad

Requires optimization for initial studies. Also expensive for only 100 samples.

The Bottom Line

Works well.

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