Protein Carbonyl Content Assay Kit ab126287 from Abcam

Product Specs
ItemProtein Carbonyl Content Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab126287
MethodFuncS
Size100 Test
Time1h 30m
SensitivityEqual to 0.015 µM

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Schwann cell Lrp1 deletion drives trigeminal neuron sensitization and orofacial pain by modulating mitochondrial function and TRPV1/TRPA1 activityJ Headache PainDecember 22, 2025Zhiting Gong, Morgan Zhang, Naijiang Liu, Kesava Asam, Stefano Martellucci, Bradley Aouizerat, Wendy Campana, Yi Yeassay Protein oxidative damage was assessed using the Protein Carbonyl Content Assay Kit (Abcam, Cat# ab126287, UK). ION and TG tissues were homogenized in RIPA lysis buffer supplemented with protease inhibitors.

(2) Cytotoxic and oxidative effects of commercially available propylene glycol (PG) and vegetable glycerin (VG): Common humectants in electronic cigarettesToxicology ReportsDecember 1, 2025Yehao Sun, Gagandeep Kaur, Felix Effah, Alan Friedman, Irfan RahmanThe protein carbonylation assay was conducted with the Protein Carbonyl Content Assay Kit (Cat. No.: ab126287, Abcam) following the manufacturer’s protocol, and the carbonylated protein content was quantified with

(3) Association Among Sperm Adiponectin, DNA Fragmentation, Oxidative Stress and Metabolites in Male InfertilityAntioxidants (Basel)November 27, 2025Rosamaria Militello, Giulia Traini, Gabriella Pinto, Tania Gamberi, Simone Luti, Sara Marchiani, Anna Illiano, Linda Vignozzi, Angela Amoresano, Alessandra ModestiMeasurement Levels of total protein oxidation were measured using the Protein carbonyl content assay kit (ab126287, Abcam). The assay provides a simple method of quantifying carbonyls groups in protein samples, as protein

(4) BCS1L ‐Associated Disease: 5′‐UTR Variant Shifts the Phenotype Towards Axonal NeuropathyAnnals of Clinical and Translational NeurologySeptember 1, 2025Rotem Orbach, Nunziata Maio, Russell J. Butterfield, A. Reghan Foley, Sarah Silverstein, Yan Li, Katherine Chao, Tanya J. Lehky, Abigail Potticary, Tracey A. Rouault, et al.instructions. Protein carbonylation was evaluated with the protein carbonyl content assay kit (Abcam, #ab126287), following the manufacturer's instructions. See also the Supplementary Appendix for additional methodological

(5) Reciprocal regulation of GPNMB/HIF-1α for Inhibition of neuronal ferroptosis in delayed encephalopathy after acute carbon monoxide poisoningActa Neuropathol CommunJuly 14, 2025Zuolong Liu, Lanyue Sun, Nan Gao, Wei Li, Li Pangiron content or ROS Protein carbonyl content was assessed using Protein Carbonyl Content Assay Kit (ab126287, Abcam). Briefly, homogenized rat hippocampal tissues were incubated with DNPH for 10 min, followed

(6) Cannabidiol Prevents Heart Failure Dysfunction and Remodeling Through Preservation of Mitochondrial Function and Calcium HandlingJACC: Basic to Translational ScienceJune 1, 2025Gerardo García-Rivas, Omar Lozano, Judith Bernal-Ramírez, Christian Silva-Platas, Felipe Salazar-Ramírez, Abraham Méndez-Fernández, Carolina Morales-Ochoa, Hugo Alves-Figueiredo, Martín Rogelio Ramos-González, Nestor Rubio-Infante, et al.markers Protein carbonyl content was assessed in homogenized cardiac tissue following the assay kit ab126287 (Abcam) instructions. Absorbance was measured was measured using a Synergy HT plate reader (BioTek). Reduced

(7) Chchd10: A Novel Metabolic Sensor Modulating Adipose Tissue HomeostasisAdvanced ScienceFebruary 22, 2025Xiaoping Wu, Zixuan Zhang, Jingjing Li, Jiuyu Zong, Lufengzi Yuan, Lingling Shu, Lai Yee Cheong, Xiaowen Huang, Mengxue Jiang, Zhihui Ping, et al.abundance of carbonylated protein in mouse eWAT was measured with Protein Carbonyl Content Assay Kit (ab126287, Abcam) according to the manufacturer's instructions. RNA Sequencing and Analysis RNA‐seq experiments

(8) Targeting sickle cell pathobiology and pain with novel transdermal curcuminPNAS NexusFebruary 13, 2025Yugal Goel, Mya A Arellano, Raghda T Fouda, Natalie R Garcia, Reina A Lomeli, Daniel Kerr, Donovan A Argueta, Mihir Gupta, Graham J Velasco, Richard Prince, et al.lysates, protein carbonyl content was assessed by a dinitrophenylhydrazine (DNPH)-based assay kit (ab126287, Abcam, Cambridge, MA, USA) (77). In these experiments, carbonyl groups in protein side chains are derivatized

(9) Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulationScientific ReportsDecember 28, 2024Wayne Hicks, Sirsendu Jana, Tigist Kassa, Richard Prince, Pedro Cabrales, Joel Friedman, Abdu I. AlayashUSA). Protein carbonyl content in RBC lysates was assessed by a dinitrophenyl hydrazine (DNPH) based assay kit (ab126287) as a measure of protein oxidation (Abcam, Cambridge, MA, USA). In these experiments, carbonyl groups

(10) High-Dimensional Single-Cell Mass Cytometry Demonstrates Differential Platelet Functional Phenotypes in Infants With Congenital Heart DiseaseArteriosclerosis, Thrombosis, and Vascular BiologyDecember 1, 2024Sean X. Gu, Brian S. Marcus, Vivian W. Gu, Adarsh P. Varghese, John Hwa, E. Vincent S. Faustinolevels of protein carbonylation by a colorimetric assay (Protein Carbonyl Content Assay Kit, Abcam, ab126287). Statistics Differences in demographics and clinical laboratory data of patients were assessed by

(11) Impact of cold storage on the oxygenation and oxidation reactions of red blood cellsFrontiers in PhysiologyAugust 19, 2024Tigist Kassa, Sirsendu Jana, Jin Hyen Baek, Abdu I. Alayashcontent was assessed as a measure of intracellular protein oxidation in RBC lysates by an assay kit (ab126287, Abcam, Cambridge, MA, United States) using dinitrophenyl hydrazine (DNPH), where carbonyl groups in

(12) Dietary Supplementation of Microalgae and/or Nanominerals Mitigate the Negative Effects of Heat Stress in Growing RabbitsBiological Trace Element ResearchAugust 1, 2024Amr M. Bashar, Sameh A. Abdelnour, Abdelhalim A. El-Darawany, Asmaa M. Sheihaab27642) were assessed based on the reaction with thiobarbituric acid [33] and protein carbonyl (PC; ab126287) were assessed using commercial ELISA kits (Abcam company) based on the reaction with DNPH [34]. Glutathione

(13) A New Tailored Nanodroplet Carrier of Astaxanthin Can Improve Its Pharmacokinetic Profile and Antioxidant and Anti-Inflammatory EfficaciesInternational Journal of Molecular SciencesJuly 18, 2024Kumudesh Mishra, Nadin Khatib, Dinorah Barasch, Pradeep Kumar, Sharon Garti, Nissim Garti, Or Kakhloninduced or not with LPS. Protein carbonylation by 2,4-dinitrophenylhydrazine was determined by AbCam’s ab126287 commercial kit as a measure of protein irreversible oxidative damage in the liver, brain, and gastrocnemius muscle

(14) Bezafibrate alleviates diabetes-induced spermatogenesis dysfunction by inhibiting inflammation and oxidative stressHeliyonMarch 30, 2024Mu Y, Luo LB, Wu SJ, Gao Y, Qin XL, Zhao J, Liu Q, Yang J.Institute (Nanjing, China). The protein carbonyl content was determined by the Protein Carbonyl Assay Kit (#ab126287, Abcam, Cambridge, MA, USA) using DNPH, according to a previous study [41]. 3.12 Determination of inflammatory

(15) Beneficial Effects of Fibroblast Growth Factor-1 on Retinal Pigment Epithelial Cells Exposed to High Glucose-Induced Damage: Alleviation of Oxidative Stress, Endoplasmic Reticulum Stress, and Enhancement of AutophagyInternational journal of molecular sciencesMarch 11, 2024Huang HW, Yang CM, Yang CH.2,4-dinitrophenylhydrazine, leading to the formation of 2,4-dinitrophenylhydrazone. The Protein Carbonyl Content Assay Kit (ab126287; Abcam, Cambridge, UK) was employed in accordance with the manufacturer’s instructions for assessing

(16) Oxidative Stress-Mediated Repression of Virulence Gene Transcription and Biofilm Formation as Antibacterial Action of Cinnamomum burmannii Essential Oil on Staphylococcus aureusInt J Mol SciMarch 6, 2024Lingling Shi, Wei Lin, Yanling Cai, Feng Chen, Qian Zhang, Dongcheng Liang, Yu Xiu, Shanzhi Lin, Boxiang Heobtained cell pellets were used to determine protein carbonyl, ROS, and MDA by using assay kits of ab126287, ab113851, and ab118970 (Abcam, Shanghai, China), respectively. The amount of ROS was given in fold

(17) Effect of Honey and Aqueous Garlic Extracts against Short-Term Exposure of Cigarette Tobacco Smoking in Mice: Histopathological and Biochemical InvestigationsJ ToxicolFebruary 19, 2024Ziad Shraideh, Darwish Badran, Ahmed Alzbeedequantified according to the instructions provided by the kit manufacturer (ab119590, Abcam, UK) and (ab126287, Abcam, UK), respectively, using a 96-well plate reader (Synergy HTX, BioTeK, USA). The evaluation of

(18) ALS-linked SOD1 mutations impair mitochondrial-derived vesicle formation and accelerate agingRedox biologyFebruary 1, 2024Guo Y, Guan T, Yu Q, Sanghai N, Shafiq K, Li M, Jiao X, Na D, Zhang G, Kong J.Antioxidant capacity test Malondialdehyde assay (ab118970, Abcam) [35] and protein carbonyl content assay (ab126287, Abcam) [36] were performed according to the manufacturer's instructions to evaluate the antioxidant

(19) Oral administration of ellagic acid mitigates perioperative neurocognitive disorders, hippocampal oxidative stress, and neuroinflammation in aged mice by restoring IGF-1 signalingScientific ReportsJanuary 30, 2024Fang Chen, Kai Lu, Ning Bai, Yabo Hao, Hui Wang, Xinrong Zhao, Fang Yueusing commercially available assay kits (MDA kit, Cat# S0131, Beyotime; carbonyl protein kit, Cat# ab126287, Abcam; SOD kit, Cat# S0101, Beyotime; CAT kit, Cat# S0051, Beyotime), stringently following the protocols

(20) FUNDC1 interacts with GPx4 to govern hepatic ferroptosis and fibrotic injury through a mitophagy-dependent mannerJournal of Advanced ResearchJanuary 1, 2024Yaguang Bi, Shuolin Liu, Xing Qin, Miyesaier Abudureyimu, Lu Wang, Rongjun Zou, Amir Ajoolabady, Wenjing Zhang, Hu Peng, Jun Ren, et al.instruction. Levels of carbonyls were quantified using a protein carbonyl content assay kit (Abcam, #ab126287). Levels of GSH, GSSG and MDA were quantified using glutathione and MDA assay kits from Beyotime (Nanjing,

Write a Review

(1)

10/7/2022Protein Carbonyl Content Assay Kit Works Ok, but Expensive Kanika JainWe wanted to analyze the levels of protein carbonylation in plasma and platelets. Worked better for plasma as compared to platelets.

International Journal of Molecular Sciences

Protein Carbonyl Content Assay Kit from Abcam

Abcam

Citations (66)

Reviews

Protein Carbonyl Content Assay Kit from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map