Phalloidin-iFluor 488 Reagent for Actin Staining

PennState College of Medicine
Pharmacology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Phalloidin-iFluor 488 Reagent

Catalog Number:

ab176753

We used this solution to visualize actin filaments in fixed mammalian cells using a confocal microscope.

Experimental Design and Results Summary

Application

Actin labelling and imaging

Starting Material

Fixed mammalian cells (COS7, HeLa) treated with 1x phalloidin stain in PBS with 1% BSA for 30 min.

Tips

Optimize the incubation time.

Results Summary

We observed very clear and bright actin filaments upon staining the cells. Background noise was very minimal. Overall it's a very good product to label and visualize actin filaments in cells.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Very specific and bright.

The Bad

None

The Bottom Line

It's a very high-quality product to visualize actin filaments. Highly recommended.

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