Easy to Use CD8 Antibody

September 20, 2022

BlueskyReddit
Immunology
UiO
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 785™ anti-human CD8 Antibody

Catalog Number:

344740

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: CD8 Antibodies

In our group, we are interested in utilizing CD8 T-cells for treatment purposes. Subsequently, we routinely isolate CD8+ T-cells from blood donors and use FACs for the analysis.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

1:100, 20 min RT

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

CD3+ cells were isolated from PBMCs and the CD8+ percentage was estimated using FACs. The antibody performs well and a clear separation of the populations isevident.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, works well.

The Bad

A bit expensive.

The Bottom Line

Like most BioLegend antibodies we are using, it works well and is reliable between batches. BV735 might not be the brighter fluorochrome, so other conjugates could be used instead, if absolute sensitivity is crucial.

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