R&D Systems
Mouse TIM-1/KIM-1/HAVCR Antibody
AF1817-SP
N/A
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We study kidney-resident macrophages and we wanted to detect tissue injury, under various inflammatory and/or infections conditions.
Immunofluorescence
PLP fixed 14 μm cryosection of mouse kidney from a 10 week old C57BL/6J mouse, infected with C. albicans
1:200 (from a stock of 0.2 mg/ml) overnight at room temperature
1% BSA, 0.3% Triton X, 2% mouse serum, 2% rat serum, 2% donkey serum
1:500 Donkey anti-goat CF488A for 1h @ room temperature
Images were acquired using an inverted Zeiss LSM780 confocal microscope with an 20x 0.8NA air objective.
The antibody yielded excellent staining of injured renal tubules (green). Based on the great staining, the primary antibody could be further diluted.
Excellent staining of KIM-1. No staining was seen on healthy kidneys.
None
Strongly recommended for the detection of KIM in murine kidney cryosections.