Great CD44 Antibody for IF

September 15, 2022

Institute of Microbiology, Infectious Diseases and Immunology
Charité – Universitätsmedizin Berlin
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 488 anti-mouse/human CD44 Antibody

Catalog Number:

103015

Clone Number:

IM7

Info:Supplier Page View Product Specs

Browse Similar Products: CD44 Antibodies

We study renal inflammation & we are interested to identify immune cell infiltration in the kidney.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed 18 μm cryosections of mouse kidney from a 10 week old C57BL/6J mouse

Primary Incubation

1:100 overnight at room temperature

Blocking Agent

1% BSA, 0.3% Triton X, 2% mouse serum, 2% rat serum

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Images were acquired using an inverted Zeiss LSM780 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Although this is an Alexa Fluor 488 conjugated antibody, the straining of CD44+ cells (green) was excellent.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great staining.

The Bad

None

The Bottom Line

Highly recommended antibody to stain CD44.

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