Good Option for Bacterial DNA Isolation

University of Texas Medical Branch Galveston

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

GenElute Bacterial Genomic DNA Kit

Catalog Number:

NA2110-1KT

Used this kit to isolate gram negative bacteria. It worked very well for the first time, but the amount of the elution buffer needed to be optimized. The amount suggested in the data sheet of the product was too much and the DNA got too diluted, so I changed to 50ul and it worked perfectly.

Experimental Design and Results Summary

Application

Gram negative bacteria DNA isolation

Starting Material

Bacteria culture

Tips

Quantity of elution buffer

Results Summary

Good DNA isolate to use for real-time PCR. Even using samples with low concentration of the bacteria, it had great results in the PCR.

DOI or PMID #

N/A

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Summary

The Good

Reliable and high quality.

The Bad

Only 70 tests per kit.

The Bottom Line

After optimization of the amount of the elution buffer has great results, great quality.

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