CD34 Used to Identify Hematomalignant Cells

Pathology
UMass
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Beckman Coulter

Product Name:

CD34-PC7, 581, 1 mL, ASR

Catalog Number:

A51077

Clone Number:

381

Info:Supplier Page 

Browse Similar Products: CD34 Antibodies

CD34 is arguably the most commonly used marker for identification of blasts in hematological malignancies. It is rarely negative, and therefore is included in almost every tube of every leukemia investigation panel. We use it to show these cells, which don't typically express other unique features. We needed a bright fluorochrome, so we went with PC7 (which is the same as PE-Cy7).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow

Primary Incubation

5uL at room temp for 10'

Blocking Agent

FBS (1%)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Beckman Coulter Navios Cytometer

Results Summary

The antibody works well, yielding a good separation of positive versus negative cells as shown in the histograms; blast cells painted red. These would've been impossible to identify based on the forward/side scatter plot (also shown).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright, easy to use, good clone.

The Bad

Expensive.

The Bottom Line

A good antibody, but comes at a price, especially when this tandem dye is easy to acquire from other suppliers.

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