High Quality Antibody Pair from Cell Signaling

August 26, 2022

Franklin and Marshall College

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb

Catalog Number:

5483

Clone Number:

D52C2

Info:Supplier Page View Product Specs

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Phospho-TBK and total TBK were detected in THP-1 cells transfected with poly dA:dT over an 8 hour time course.

Experimental Design and Results Summary

Applications

Western Blot

Sample

THP-1 cells stimulated with poly dA:dT

Primary Incubation

1:5000 in TBST+5% milk overnight at 4C

Blocking Agent

TBST +5% milk for 1 hour at room temperature

Secondary Incubation

1:10000 goat anti-rabbit HRP (Bio-Rad)

Tertiary Incubation

None

Detection

Clarity max ECL

Results Summary

This antibody detected an inducible protein at the predicted molecular weight. Total TBK was used as an internal control.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well.

The Bad

Expensive.

The Bottom Line

A good choice, but it is expensive.

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