Miltenyi Biotec
CD79b Antibody, anti-mouse, APC-Vio® 770, REAfinity™
130-119-429
REA1117
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment, we are looking for markers of PC.
Flow Cytometry
Mouse splenocytes
Surface staining with CD79b APC-Vio® 770, 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
In this work, we were looking for surface markers that are differentially expressed by B cells (CD19+) and plasma cells (PC). Here, we were titrating anti-CD79b which is express in B cells. We conclude that this antibody should be used at 1/100.
It works well, and it is affordable.
Antibody has a good signal.