Universal Colorimetric PARP Assay Kit - Histone-Coated Wells 4677-096-K from R&D Systems, a Bio-Techne Brand

Product Specs
ItemUniversal Colorimetric PARP Assay Kit - Histone-Coated Wells
CompanyR&D Systems, a Bio-Techne Brand
Price $566.00Supplier Page View Company Product Page
Catalog Number4677-096-K
Quantity1 Kit
ReactivityMulti-Species
Detection TargetPARP
StorageThe product requires storage at both -20 to -70 °C and 2 - 8 °C. Consult the product insert for specific storage temperatures. Do not use past expiration date
NCBI Full Gene Namepoly(ADP-ribose) polymerase 1
NCBI Gene AliasesADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1

Specifications/Features

Available conjugates: Sizes Available: 1 Kit

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) VEGF inhibitor‐induced vascular dysfunction involves redox‐sensitive PARP activation and SIRT1 disruptionExp PhysiolJune 17, 2026Karla B. Neves, Rheure Alves‐Lopes, Augusto C. Montezano, Rhian M. Touyzdetection of biotinylated poly (ADP‐ribose) deposited by PARP‐1 onto immobilized histones (R&D Systems® 4677‐096‐K). Forty micrograms of of protein from cell lysates was loaded into a 96‐well plate coated with histones

(2) Drug repurposing screen targeting PARP identifies cytotoxic activity of efavirenz in high-grade serous ovarian cancerMol Ther OncolDecember 19, 2024Matthews B, Wong-Brown M, Liu D, Yee C, Dickson KA, Schneider J, Islam S, Head R, Martin JH, Ford CE, et al.(COV362, OVSAHO, and TYK-nu) were measured using a PARP/Apoptosis Universal Colorimetric Assay (cat. #4677-096-K, R&D Systems, Minneapolis, MN, USA) as per the manufacturer’s protocol. Briefly, cell pellets were collected

(3) Functional identification of soluble uric acid as an endogenous inhibitor of CD38eLifeNovember 11, 2024Wen S, Arakawa H, Yokoyama S, Shirasaka Y, Higashida H, Tamai I.,CycLex NAMPT Colorimetric Assay Kit Ver.2,MBL,Cat#CY-1251V2, Commercial assay or kit,HT Universal Colorimetric PARP Assay Kit,R&D Systems,Cat#4677-096-K, Cell line (human),A549,ATCC,CCL-185, Cell line (human),THP-1,ATCC,TIB-202, Cell line(mouse),ICR

(4) DNA repair and anti-cancer mechanisms in the long-lived bowhead whalebioRxiv : the preprint server for biologyNovember 5, 2024Firsanov D, Zacher M, Tian X, Sformo TL, Zhao Y, Tombline G, Lu JY, Zheng Z, Perelli L, Gurreri E, et al.

(5) Design, synthesis and development of a dual inhibitor of Topoisomerase 1 and poly (ADP-ribose) polymerase 1 for efficient killing of cancer cellsEuropean journal of medicinal chemistryOctober 5, 2023Guha Majumdar A, Shree S, Das A, Kumar BK, Dey P, Subramanian M, Patro BS.

(6) A Bifunctional PARP-HDAC Inhibitor with Activity in Ewing SarcomaClinical cancer research : an official journal of the American Association for Cancer ResearchSeptember 1, 2023Ramos L, Truong S, Zhai B, Joshi J, Ghaidi F, Lizardo MM, Shyp T, Kung SHY, Rezakhanlou AM, Oo HZ, et al.activity was measured using the HT Universal Colorimetric PARP assay kit (R&D Systems, catalog No. 4677–096-K), and PARP2 activity was measured using the PARP2 colorimetric assay kit (BPS Bioscience, catalog No.

(7) NAD depletion mediates cytotoxicity in human neurons with autophagy deficiencyCell reportsMay 30, 2023Sun C, Seranova E, Cohen MA, Chipara M, Roberts J, Astuti D, Palhegyi AM, Acharjee A, Sedlackova L, Kataura T, et al.Plus TUNEL Assay Kit,Invitrogen,Cat# C10617 HT Universal Colorimetric PARP Assay Kit,R&D Systems,Cat# 4677-096-K Mitochondria Isolation Kit for Cultured Cells,ThermoFisher,Cat# 89874 NAD/NADH Quantitation Colorimetric Kit

(8) Role of PARP and TRPM2 in VEGF Inhibitor-Induced Vascular DysfunctionJournal of the American Heart AssociationFebruary 21, 2023Neves KB, Alves-Lopes R, Montezano AC, Touyz RM.absence of pharmacological inhibitors by a colorimetric assay according to the manufacturer's protocol (4677‐096‐K, R&D Systems). Results were normalized to the protein concentration. Statistical Analysis For vascular

(9) Natural variations of ZmSRO1d modulate the trade-off between drought resistance and yield by affecting ZmRBOHC-mediated stomatal ROS production in maizeMolecular plantOctober 3, 2022Gao H, Cui J, Liu S, Wang S, Lian Y, Bai Y, Zhu T, Wu H, Wang Y, Yang S, et al.

(10) Inhibition of nonhomologous end joining‐mediated DNA repair enhances anti‐HBV CRISPR therapyHepatology CommunicationsSeptember 1, 2022Kazuhiro Murai et al.DOX for Cas9 expression, PARP activity was measured with a Poly (ADP‐Ribose) Polymerase Assay Kit (4677‐096‐K; Bio‐Techne) according to the manufacturer's protocol. Statistical analysis The data are presented

(11) BRCA mutations lead to XIAP overexpression and sensitise ovarian cancer to inhibitor of apoptosis (IAP) family inhibitorsBritish journal of cancerAugust 1, 2022Cremona M, Vandenberg CJ, Farrelly AM, Madden SF, Morgan C, Kalachand R, McAlpine JN, Toomey S, Huntsman DG, Grogan L, et al.

(12) SIK2 inhibition enhances PARP inhibitor activity synergistically in ovarian and triple-negative breast cancersThe Journal of clinical investigationJune 1, 2022Lu Z, Mao W, Yang H, Santiago-O'Farrill JM, Rask PJ, Mondal J, Chen H, Ivan C, Liu X, Liu CG, et al.assay. PARP enzyme activity was measured using a PARP universal colorimetric assay kit (R&D Systems, 4677-096-K). Cells from different ovarian cancer cell lines were plated and treated with ARN3236 (6 μM) or ARN3261

(13) Development of 1-(4-(Substituted)piperazin-1-yl)-2-((2-((4-methoxybenzyl)thio)pyrimidin-4-yl)oxy)ethanones That Target Poly (ADP-Ribose) Polymerase in Human Breast Cancer CellsMoleculesApril 29, 2022Suresha N. Deveshegowda, Prashant K. Metri, Rashmi Shivakumar, Ji-Rui Yang, Shobith Rangappa, Ananda Swamynayaka, Muthu K. Shanmugam, Omantheswara Nagaraja, Mahendra Madegowda, Priya Babu Shubha, et al.catalytic activity of PARP1 was determined using the PARP/Apoptosis Universal Colorimetric Assay Kit (4677-096-K, R&D Systems Ltd., Abingdon, UK) [46]. Compounds 5a or 5e or Olaparib were added to recombinant human

(14) TET-mediated DNA hydroxymethylation is negatively influenced by the PARP-dependent PARylationEpigenetics & chromatinApril 5, 2022Tolić A, Ravichandran M, Rajić J, Đorđević M, Đorđević M, Dinić S, Grdović N, Jovanović JA, Mihailović M, Nestorović N, et al.

(15) Design and Activity of Novel Oxadiazole Based Compounds That Target Poly(ADP-ribose) PolymeraseMoleculesJanuary 21, 2022Divakar Vishwanath, Swamy S. Girimanchanaika, Dukanya Dukanya, Shobith Rangappa, Ji-Rui Yang, Vijay Pandey, Peter E. Lobie, Basappa BasappaPARP Activity PARP activity was measured using the PARP/Apoptosis Universal Colorimetric Assay Kit (4677-096-K, R&D Systems Ltd., Abingdon, UK) [45]. The enzyme inhibition assay uses the histone proteins immobilized

(16) JAK Inhibition Prevents DNA Damage and Apoptosis in Testicular Ischemia-Reperfusion Injury via Modulation of the ATM/ATR/Chk PathwayInternational journal of molecular sciencesDecember 13, 2021Khashab F, Al-Saleh F, Al-Kandari N, Fadel F, Al-Maghrebi M.

(17) Cytocidal Antitumor Effects against Human Ovarian Cancer Cells Induced by B-Lactam Steroid Alkylators with Targeted Activity against Poly (ADP-Ribose) Polymerase (PARP) Enzymes in a Cell-Free AssayBiomedicinesAugust 17, 2021Nikoleousakos N, Dalezis P, Polonifi A, Geromichalou EG, Sagredou S, Alifieris CE, Deligiorgi MV, Sarli V, Trafalis DT.

(18) TSL-1502, a glucuronide prodrug of a poly (ADP-ribose) polymerase (PARP) inhibitor, exhibits potent anti-tumor activity in preclinical modelsAmerican journal of cancer researchJanuary 1, 2021Wang L, Zhu X, Li L, Li L, Fu L, Li Y, Fu H, Chen X, Lou L.

(19) Inhibition of NADPH oxidase alleviates germ cell apoptosis and ER stress during testicular ischemia reperfusion injurySaudi journal of biological sciencesAugust 1, 2020Al-Saleh F, Khashab F, Fadel F, Al-Kandari N, Al-Maghrebi M.

(20) Riluzole protects against skeletal muscle ischaemia-reperfusion injury in a porcine modelInjuryFebruary 1, 2020Li RW, Deng Y, Pham HN, Weiss S, Chen M, Smith PN.

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7/12/2022Reliable and Precise IC50 Values Arianna NapoliIn our lab we study PARP-1 that is a nuclear protein and mediates Poly-ADP-ribosylation o nuclear proteins (as it is, and histones for example), a process that occurs in response to DNA damage. ... Read Review

Universal Colorimetric PARP Assay Kit - Histone-Coated Wells

Universal Colorimetric PARP Assay Kit - Histone-Coated Wells

Universal Colorimetric PARP Assay Kit - Histone-Coated Wells

Universal Colorimetric PARP Assay Kit - Histone-Coated Wells from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (49)

Reviews

Universal Colorimetric PARP Assay Kit - Histone-Coated Wells from R&D Systems, a Bio-Techne Brand

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