Transferrin Receptor Monoclonal Antibody

June 13, 2022

Biology of Aging
MC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Transferrin Receptor Monoclonal Antibody

Catalog Number:

13-6800

Clone Number:

H68.4

Transferrin Receptor (CD71) antibody was used for western blot analysis of HBMECs lysates. Although there is more than one band, the expression at 90 kDa is clear.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human endothelial cells

Primary Incubation

1:500, overnight@4ºC

Blocking Agent

5% BSA/TBS

Secondary Incubation

Anti-mouse IgG HRP (#HAF007, R&D systems)

Tertiary Incubation

N/A

Detection

Chemiluminescent

Results Summary

Protein loading: 25 ug/mL.There is a clear band at 90 kDa, although it is also visible another band at higher molecular weight.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Straightforward.

The Bad

N/A

The Bottom Line

The antibody has great signal with low exposure.

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