BD Biosciences
BV750 Mouse Anti-C3/C3b/iC3b
752717
6C9
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BV750 Mouse Anti-C3/C3b/iC3b recognizes the components and fragments of complement C3, which may be present on the platelet surface in certain pathologic states. Bright C3b staining on human platelets.
Flow Cytometry
Human whole blood collected in sodium citrate
30 min at room temperature
Staining buffer contains 0.5% BSA
N/A
Fluorescence
Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing BV750 Mouse Anti-C3/C3b/iC3b with or without 10 µg/mL Anti-GPIIb/IIIa [10E5] (complement activator). 10E5-treated platelets show bright staining for complement C3.
Bright C3b staining on human platelets.
BV750 Mouse Anti-C3/C3b/iC3b allows bright staining of complement components and fragments on the surface of human platelets.