Excellent GAPDH Antibody

May 24, 2022

BlueskyReddit
Nanomedicine
Houston Methodist Research Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling Technology (CST)

Product Name:

GAPDH XP® Rabbit mAb

Catalog Number:

5174S

Clone Number:

D16H11

Info:Supplier Page View Product Specs

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I have been using this GAPDH antibody for 7 years and very satisfied with its performance as a loading control in Western Blot. I tested in human/rat/mice cells. The results always looks great.

Experimental Design and Results Summary

Applications

Western Blot

Sample

A549 Human Lung Cancer Cell Line

Primary Incubation

1:5000

Blocking Agent

5% milk

Secondary Incubation

1:5000 room temperature 2 h

Tertiary Incubation

N/A

Detection

ChemiDoc Imaging Systems

Results Summary

The bands look great.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use and reliable.

The Bad

N/A

The Bottom Line

This is an excellent GAPDH antibody. It works with human/mice/rat/monkey. Would buy again and again.

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