Excellent Antibody to Detect ß-Actin

May 13, 2022

BlueskyReddit
Pharmacology
PennState College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

β-Actin (D6A8) Rabbit mAb

Catalog Number:

8457S

Clone Number:

N/A

We used this antibody to detect β-Actin levels in mammalian cell lysates. The antibody works really well. Specificity is really good. Highly recommended.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mammalian cell line whole cell lysate

Primary Incubation

1:2000, overnight incubation at 4 degree Celsius

Blocking Agent

5% BSA in TBST

Secondary Incubation

Goat anti-rabbit IgG HRP Ab, dilution (1:2500) in 5% BSA , 1hr at RT

Tertiary Incubation

N/A

Detection

ECL

Results Summary

We observed a clear, very specific bright actin band at the expected size. Results are highly consistent. Very less non-specificity.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Strong clear band, highly specific.

The Bad

None

The Bottom Line

A must-have antibody for beta-actin expression studies.

Share your experience with other scientists. Write a Review! »

Join the discussion