beta-Actin (D6A8) Rabbit mAb 8457S from Cell Signaling Technology

Product Specs
Itembeta-Actin (D6A8) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number8457S
Quantity100 µl
ApplicationsW, IF-IC, FC-FP
ReactivityH, M, R, Mk, Dm, Z
Targetbeta-Actin
HostRabbit
NCBI Gene AliasesBRWS1, PS1TP5BP1
ClonalityMonoclonal
NCBI Full Gene Nameactin beta
Research AreaCytoskeletal Signaling
IsotypeIgG
CloneD6A8
Molecular Weight45

Questions and Answers

Can this β-Actin antibody be used for western blotting in my cancer research?

Yes, the β-Actin (D6A8) Rabbit mAb has been successfully utilized in western blotting, as evidenced by its application in various studies, including those investigating the effects of different treatments on cancer cells, such as in the research by and .

Is this antibody suitable for detecting β-Actin in non-human primates or zebrafish?

The β-Actin (D6A8) Rabbit mAb is reactive with multiple species, including non-human primates and zebrafish, making it suitable for studies involving these organisms.

How does this antibody perform in immunocytochemistry applications?

The β-Actin (D6A8) Rabbit mAb has been shown to work effectively in immunocytochemistry, as indicated by its use in studies that require visualization of β-Actin in various cell types, including those in cancer research contexts.

Does this antibody cross-react with other actin isoforms?

While the β-Actin (D6A8) Rabbit mAb specifically recognizes β-actin, it may cross-react with cytoplasmic γ-actin due to the high sequence identity between these isoforms. However, it does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms.

Cell Signaling Technology

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Phone: 978-867-2388
Fax: 866-432-6112
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Oridonin-Loaded PDA@Gel@GO Nanocapsules Modulate NLRP3 and Epithelial Repair in ColitisFASEB JApril 30, 2026Wenbo Yuan, Qirui Zhao, Jing Hua, Lingyao Xu, Yufan Wang, Tongtong Xu, Zuchao Du, Liping Wu, Daxiang Cuiab180799, UK), Caspase‐1 (Abcam, ab207802, UK), IL‐1β (Abcam, ab254360, UK), and β‐actin (Cell Signaling, #8457, USA), each diluted 1:1000. After washing, membranes were incubated at ambient temperature for 1 h with

(2) Trans-Chalcone Reduces Inflammation and Pain Triggered by Superoxide Anion: Neuronal and Non-Neuronal MechanismsJ Inflamm ResApril 25, 2026Maiara Piva, Marília F Manchope, Fernanda Barbosa-Costa, Beatriz H S Bianchini, Ketlem C Andrade, Letícia Coelho Silva, Cássia Calixto-Campos, Fernanda S Rasquel-Oliveira, Victor Fattori, Ana Carla Zarpelon-Schutz, et al.Santa Cruz Biotechnology, #sc-136548,) and β-actin rabbit monoclonal (1:1000, D6A8, Cell Signaling, #8457), and secondary antibodies goat anti-mouse IgG Alexa Fluor™ 488 (1:200, Invitrogen, #A11001) and goat

(3) Nebivolol suppresses glioblastoma progression via dual modulation of mitochondrial metabolism and AKT/mTOR/4EBP1 signaling axisPLoS GenetApril 24, 2026Lingni Zhou, Hongyu Che, Hongyan Jiang, Luxin Yin, Yongang Jiang, Yuhui Zhang, Haoran Liang, Rutong Yu, Xu Zhang, Xuejiao Liu(#4058), p-mTOR (#5536), mTOR (#2983), p-4EBP1 (#2855), 4EBP1 (#9644), GAPDH (#5174) and β-actin (#8457) primary antibodies were purchased from Cell Signaling Technology (CST, MA, USA). CCK-8 assay Cell

(4) Stemness and Survival: CD117+/CD133+ Subpopulations Sustain PI3K Signaling and Drive Imatinib Resistance in Head and Neck Mucosal MelanomaCellsApril 19, 2026Sofie-Yasmin Hassan, Simeon Santourlidis, Thomas W. Flanagan, Sarah-Lilly Hassan, He Zhou, Morna F. Schmidt, Claudio Cacchi, Matthias Ferdinand Lammert, Mossad Megahed, Amir Sadegh Yazdi, et al.CD133 [(D2V8Q) XP® Rabbit mAb #64326], anti-CD133 (#64326), c-Kit (#37805), p85 (#4257), and Actin (#8457), and Phospho-PDK1 [Ser241] (#3061) were all purchased from Cell Signaling Technology (Beverly, MA,

(5) Innate immune sensing of dietary alcohol ignites inflammation to drive alcohol-related diseaseSci AdvApril 10, 2026Yeonseo Jang, Hoeun Bae, SuHyeon Oh, Gyeongju Yu, Hyun Bae, Minh Quan Nguyen, Raghvendra Mall, Minjie Fu, Aritra Ghosh, Jihye Lee, et al.4511L), ADAR1 (1:250; Santa Cruz, sc-73408), ISG15 (1:250; Santa Cruz, sc-166755), β-actin (1:1000; CST, 8457S), and glyceraldehyde phosphate dehydrogenase (GAPDH; 1:50,000; Proteintech, 60004-1-lg and 1:1000; CST,

(6) In Silico and In Vitro Investigation of Apoptosis-Mediated Antiproliferative Activity of Capsaicin and Alpha-Lipoic Acid Against Prostate Cancer CellsCurr Issues Mol BiolApril 4, 2026Ogunc Meral, Burcu Menekse Balkan, Dilek Nur Bestil, Serkan Sayiner, Deniz Ceylanli, Sare Uyurca, Sinem Pehlivan, Guzin Ozkurt, Gorkem Kismali, Tevhide SelCell Signalling Technology, USA, 1:1000 dilution) were used as a primary antibody and β-actin (Cat. No: 8457, Cell Signalling Technology, USA, 1:1000 dilution) was used as a loading control to normalize the levels

(7) Corylin inhibits liver cancer cell growth through the endogenous apoptosis and mitophagyTranslational Cancer ResearchMarch 31, 2026Renshuang Zhao, Xin Wang, Mingming Cao, Yaru Li, Shanzhi Li, Zhiru Xiu, Yilong Zhu, Yiquan Li3-phosphate dehydrogenase (GAPDH) (Cell Signaling, Danvers, MA, USA; 5174), anti-beta-actin (Cell Signaling; 8457), anti-E-cadherin (Cell Signaling; 3195), anti-N-cadherin (Cell Signaling; 13116), anti-vimentin (Cell Signaling

(8) RNA N6-methyladenosine (m6A) regulates cell cycle progression in diffuse midline glioma (DMG) and confers sensitivity to FTO inhibitionCell Death & DiseaseMarch 26, 2026Samuel E. Ross, Holly Holliday, Eyden Wang, Mahdi Zeraati, Maria Tsoli, David S. Ziegler, Marcel E. Dinger#4513, p-Rb S807/811 #8526, total Rb #9039, p21 #2947, cleaved caspase 3 #9664, GAPDH #2118, β-actin #8457). Following washing, membranes were incubated in horseradish peroxidase- conjugated secondary antibody

(9) Redox cycling nitroxide limits cellular iron availability and selectively inhibits iron-sulfur cluster metabolismCell Death DiscoveryMarch 24, 2026Erdem M. Terzi, Kenji M. Fujihara, Marte Molenaars, Douglas E. Biancur, Richard Possematoairways. Methods Reagents Reagents were obtained from the following sources. Antibodies: β-actin (8457), FTH1 (3998), IRP1 (20272), IRP2 (37135), and TFR1 (CD71, 13208) were from Cell Signaling Technologies;

(10) Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeresCell ReportsMarch 24, 2026Erin R. Taylor, Bruce Proctor, Melina Vaurs, Guanhui Wu, Manon Mahieu, Shreya Shrestha, Daniel Morrison, Laila Weatherly, Shane Brelinsky, Maya Raghunandan, et al.antibody,Cell Signaling Technology,Cat# 2276; RRID: AB_331783 β-Actin antibody,Cell Signaling Technology,Cat# 8457; RRID: AB_10950489 H3K9me3 antibody,Abcam,Cat# ab8898; RRID: AB_306848 Histone H3 antibody,Abcam,Cat#

(11) KNL1 Regulates Ferroptosis Resistance and Migration in Lung Adenocarcinoma Cells via AMPK-mTOR SignalingOncology ResearchMarch 23, 2026Yiran Dong, Jingyue Wang, Jiayang Chen, Liang Mo, Yong YouPenicillin-Streptomycin(Gibco,15140122); KNL1 antibody (CST, # 26687, Danver, MA, USA, 1:1000); β-actin antibody (CST, #8457S, 1:1000); Slug antibody (CST, # 9585, 1:1000); Vimentin antibody (CST, # 5741, 1:1000); xCT antibody

(12) FOXM1-specific TCR-engineered T cells target non-small cell lung cancerCancer Immunology ResearchMarch 19, 2026Emily Bontekoe, Minying Zhang, Peixin Jiang, Amanda Montoya, Yulia Shulga, Jared K. Slone, Emily Paul, Christopher Polera, Sarah Forward, Barbara Nassif Nassif Rausseo, et al.(Bio-Rad, 1704157). Membranes were probed with FOXM1 and normalized to β-actin (Cell Signaling Technology, 8457T) following standard protocols. Proteins were detected using enhanced chemiluminescence reagents (Supersignal West Femto Maximum Sensitivity Substrate

(13) Epigenetic context defines the transcriptional activity of canonical and noncanonical NF-κB signaling in pancreatic cancerCell Death DiscoveryMarch 17, 2026Joana E. Aggrey-Fynn, Joshua Busch, Dominik Saul, Ashish Rajput, Kerstin Willecke, Meghana Manjunath, Nicole Klimt, Kothai Rajendran, Nadine Schacherer, Wanwan Ge, et al.Cell Signaling Technology; 13586; RRID:AB_2665516), and β-Actin (D6A8) (1:1000; Cell Signaling Technology; 8457; RRID:AB_10950489). Secondary antibodies were used at a 1:2500 dilution and included goat anti-rabbit IgG Starbright Blue 700

(14) Microbial metabolism of methotrexate produces a STAT3 signaling molecule that alleviates gut inflammationbioRxivMarch 14, 2026Juyeong Cho, Rebecca Danner, Woo Sung Kim, Jonathan Williams, Anshit Singh, Jiyoung A. Han, Jonathan G. Van Vranken, Allison S. Walker, Timothy Rhoads, Snehal N. Chaudhari,Cell Signaling Technology,"Cat #9134, RRID: AB_331589" Rabbit β-Actin Antibody,Cell Signaling Technology,"Cat #8457, RRID: AB_10950489" Mouse anti-rabbit IgG-HRP,Santa Cruz Biotechnology,"Cat #sc-2357, RRID: AB_628497" "Goat Anti-Rabbit IgG (H+L) Antibody

(15) Cell type-specific epigenetic regulatory circuitry of coronary artery disease lociNat CommunMarch 10, 2026Dennis Hecker, Xiaoning Song, Nina Baumgarten, Anastasiia Diagel, Nikoletta Katsaouni, Ling Li, Shuangyue Li, Ranjan Kumar Maji, Fatemeh Behjati Ardakani, Lijiang Ma, et al.1:500, #STJ194004, Lot: 4RC229RC22, St John’s Laboratory, London, UK) or β-actin (dilution 1:1000, #8457S, Lot: 8, Cell Signaling Technology, Danvers, Massachusetts, USA). After washing, membranes were incubated

(16) High-throughput screening identifies a trafficking corrector for long QT syndrome–associated KCNQ1 variantsJCI InsightMarch 9, 2026Katherine R. Clowes Clowes Moster, Carlos G. Vanoye, Ana C. Chang-Gonzalez, Ian M. Romaine, Katherine M. Stefanski, Mason C. Wilkinson, Joshua A. Bauer, Thomas P. Hasaka, Emily L. Days, Reshma R. Desai, et al.(Alomone Labs, APC-022), and the bottom half was probed with an anti–β-actin antibody (Cell Signaling, 8457S) as a loading control. Bands corresponding to mycKCNQ1 (approximately 70 kDa) and β-actin were quantified

(17) NF-κB activation in astrocytes impairs wound healing after traumatic brain injury in male miceNat CommunMarch 6, 2026Tabea M. Hein, Ester Nespoli, Marsela Hakani, Heinrich Wendt, Stephanie Nadine May, Jasmin Jorzik, Duygu Yagdiran, Judith S. Schlett, Konstantinos Tsesmelis, Miltiadis Tsesmelis, et al.mAb (1:1000, Cell Signaling Technology #8943), β-Actin rabbit mAb (1:500, Cell Signaling Technology #8457), HRP-linked anti-rabbit IgG (1:2000, Cell Signaling Technology #7074), anti-EAAT1 rabbit mAb (1:1000,

(18) Attenuation of cGAS-STING signaling-mediated lung inflammation during infection through autophagy induction by bioactive nanodevicesTheranosticsMarch 4, 2026Mimi Pang, Xiang Wang, Zichen Song, Rujing Lin, Sixia Liu, Man Xing, Wenfei Xu, Jiameng Gong, Ying Qi, Mei Du, et al.IκBα (#9242), phosphorylated-AMPKα (p-AMPKα, #2535), SQSTM1/p62 (#39749), LC3B (#43566), β-actin (#8457), and GAPDH (#2118) as well as the HRP-conjugated anti-rabbit (#7074) or anti-mouse (#7076) antibodies

(19) USP35 Acts as a Deubiquitinating Enzyme for ID3 to Promote Immune Escape in Colorectal CancerAdvanced ScienceMarch 1, 2026Wenxin Chen, Ling Wang, Hongmei Fan, Lisheng Li, Lingyu Zhang, Ruoxin Li, Fang Liu, Fuli Wen, Yunbin Ye, Chuanzhong Huang#SAB4301882), Ubiquitin (CST #3933), Flag‐tag (Sigma #F9291), HA‐tag (Sigma #H9658), β‐actin (CST #8457), USP14 (CST #11931T), SLC7A11 (Proteintech #26864‐1‐AP). 5.7 Protein Half‐Life Detection Assay CRC

(20) A cell-based assay for retinaldehyde dehydrogenase activity: Retinoid quantification as an alternative to current fluorescence-based approachesJournal of Biological ChemistryMarch 1, 2026Julie Charpentier, Yan Lu, Serena Gallozzi, Shubhangi Seth, Mark P. Hodson, James R. Krycer, Severine Navarropreviously (38). Primary antibodies detecting RALDH2 (catalogue #83805) and β-actin (clone D6A8, catalogue #8457) were obtained from Cell Signaling Technology. Detection was performed using either (i) chemiluminescence

Write a Review

(1)

5/13/2022Excellent Antibody to Detect ß-Actin Yashavantha VishweshwaraiahWe used this antibody to detect ß-Actin levels in mammalian cell lysates. The antibody works really well. Specificity is really good. Highly recommended.
(1)

5/2/2019Okay Antibody to Detect Actin for Loading Chirag PatelNice antibody to detect beta-actin as a loading control with specificity however not reliable after reusing it.
(1)

12/13/2018Decent Antibody to Detect Actin for LoadingDr. Min Hee OhWe routinely perform western blots for all types of cells (macrophages, tumor, T cells). To normalize total protein content in certain experiments, we use actin as a control in our blots. This is a ... Read Review

beta-Actin (D6A8) Rabbit mAb

beta-Actin (D6A8) Rabbit mAb from Cell Signaling Technology

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beta-Actin (D6A8) Rabbit mAb from Cell Signaling Technology

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