Excellent GATA-3 Antibody

School of Natural Science
NUIG
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

GATA-3 (D13C9) XP® Rabbit mAb

Catalog Number:

5852

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: GATA-3 Antibodies

This antibody from Cell Signaling Technology specifically detects the transcription factor GATA-3. It has a very sharp band and works very well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line, MDA-MB-231

Primary Incubation

1:1000. Overnight. Cold room.

Blocking Agent

5% Milk in PBS

Secondary Incubation

1:7000. 1 hour at room temperature.

Tertiary Incubation

N/A

Detection

Bioluminescence (HRP-conjugated)

Results Summary

GATA-3 was overexpressed in MDA-MB-231 cells. A stronger band is detected in GATA3 overexpression than the empty vector (E.V.).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No unspecific bands detected.

The Bad

Quite costly although very good antibody.

The Bottom Line

Excellent antibody.

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