Great Antibody for Loading Control and PLA Control

May 10, 2022

BlueskyReddit
Neurosurgery
Penn State College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Vinculin antibody

Catalog Number:

ab91459

Clone Number:

N/A

Vinculin antibody used for proximity ligation assay and works great on HEK 293 cells at 1:200 dilution.

Experimental Design and Results Summary

Applications

Proximity Ligation Assay

Sample

HEK 293 cells

Primary Incubation

1:200

Blocking Agent

DuoLink Blocking buffer

Secondary Incubation

DuoLink Probes

Tertiary Incubation

N/A

Detection

DuoLink Reagent Red

Results Summary

Used in combination with actin as a positive control for PLA.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

PLA control

The Bad

No cons.

The Bottom Line

Recommended for all applications.

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