Best Antibody for PLA

May 02, 2022

BlueskyReddit
Neurosurgery
Penn State College of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

ProteinTech

Product Name:

Transferrin antibody

Catalog Number:

66161-1-1g

Clone Number:

N/A

This antibody was used for proximity ligation assay and works great on HEK 293 cells at 1:200 dilution.

Experimental Design and Results Summary

Applications

Proximity Ligation Assay

Sample

HEK 293 cells

Primary Incubation

1:200

Blocking Agent

DuoLink Blocking buffer

Secondary Incubation

DuoLink Probes

Tertiary Incubation

N/A

Detection

DuoLink Reagent Red

Results Summary

Used to demonstrate the interaction of transferrin and TfR for positive control.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Amazing for PLA, cheap

The Bad

No cons.

The Bottom Line

I highly recommend for PLA.

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