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SIRPα (CD172a)
144028
P84
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We are interested to study tissue-resident macrophages in various organs. As a positive control for SIRPα we stained the spleen.
Immunofluorescence
PLP fixed 14 μm cryosections of mouse spleen from a 10 week old C57BL/6J mouse
1:100 overnight at room temperature
1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum
N/A
Images were acquired using an inverted Zeiss LSM780 confocal microscope with an 20x 0.8NA air objective.
Excellent staining of SIRPα+ cells
Very nice staining with minimal background.
None
Highly recommended.