Efficient FceR1 Antibody for EMSA

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen

Product Name:

FceR1 alpha Polyclonal Antibody

Catalog Number:

PA5-115221

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: FCER1A Antibodies

FcεRI is one of the high-affinity receptor for the Fc region of immunoglobulin E. It is a tetrameric receptor complex that binds Fc portion of the ε heavy chain of IgE. It is highly expressed on mast cells and basophils and is inducible in eosinophils. FcεRI controls the production of important immune mediators and promote inflammation. The most known mediator is histamine, which results in heat, swelling, pain, redness and loss of function. Here we checked the time course effect of FcεRI expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:500 in 1x TBST prepared in skim milk

Blocking Agent

5% TBST prepared in skim milk

Secondary Incubation

1:4000 in 1x TBST prepared in skim milk

Tertiary Incubation

None

Detection

ECL

Results Summary

We found no change in FceR1 alpha expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This is a high concentration antibody and works great for EMSA

The Bad

None

The Bottom Line

Works great and very specific. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion