Cell Signaling
HIF-1α (D1S7W) XP® Rabbit mAb #36169
D1S7W
N/A
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I used this antibody with the aim to study neurodegenerative disorders genesis in vitro. My sample were SHSY5Y cells induced with amyloid beta 42. I used a dilution 1:1000 in 5% BSA and a secondary antibody diluted 1:5000 for 2 hours. Predicted molecular weight was accurate and quality of Western Blot bands was very high. I definitely recommend the product and the conditions suggested by the vendor.
Western Blot
SH-SY5Y
1:1000 overnight
5% BSA in TBST 1x
1:5000 2 hours
High quality and specificity
Specificity
Nothing to complain about.