Excellent HIF-1a Antibody

Nanomedicine
HMRI
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

HIF-1α (D1S7W) XP® Rabbit mAb #36169

Catalog Number:

D1S7W

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: HIF-1A Antibodies

I used this antibody with the aim to study neurodegenerative disorders genesis in vitro. My sample were SHSY5Y cells induced with amyloid beta 42. I used a dilution 1:1000 in 5% BSA and a secondary antibody diluted 1:5000 for 2 hours. Predicted molecular weight was accurate and quality of Western Blot bands was very high. I definitely recommend the product and the conditions suggested by the vendor.

Experimental Design and Results Summary

Applications

Western Blot

Sample

SH-SY5Y

Primary Incubation

1:1000 overnight

Blocking Agent

5% BSA in TBST 1x

Secondary Incubation

1:5000 2 hours

Tertiary Incubation

N/A

Detection

Western Blot

Results Summary

High quality and specificity

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Specificity

The Bad

Nothing to complain about.

The Bottom Line

High quality and specificity

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