Excellent Antibody for Effector Memory T Cells

April 21, 2022

Chemical/Biomedical Engineering
University of Toronto
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Cx3cr1 BV711

Catalog Number:

149031

Clone Number:

SA011F11

Info:Supplier Page View Product Specs

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We are interested in using cutting edge single cell analysis methods (spectral flow cytometry, scRNAseq, etc) to study the mechanisms responsible for establishing and maintaining immune tolerance.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Lymph node, spleen

Primary Incubation

Block: 1:50 in 100uL at 37C for 30 min

Blocking Agent

Fc Block (aCD16/32)

Secondary Incubation

L/D: Zombie NIR 1:4000 in 100 uL at RT for 20 min

Tertiary Incubation

Surface stain: 1:500 in 100uL at 4C for 30 min

Detection

Spectral flow cytometry (Cytek Aurora)

Results Summary

This antibody stains many different immune cell populations with varying strength including microglia, monocytes, and dendritic cells but has especially strong staining for a relatively rare subset of T effector memory T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great for studying rare immune cell subsets.

The Bad

Stains many different types of immune cells.

The Bottom Line

Great antibody for studying the immune system.

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