Biolegend
PE anti-mouse CD130 (gp130) Antibody
149403
4H1B35
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we were looking for surface markers of PCs.
Flow Cytometry
Mouse spleen
Surface staining with CD130 PE (1/100), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
We found that PCs (CD138hi) express low amounts of CD130.
https://doi.org/10.3389/fimmu.2022.873217
It works.
It is not bright.
It can be used carefully.