Relatively Good Anti-Mouse CD172a

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Dazzle™ 594 anti-mouse CD172a (SIRPα) Antibody

Catalog Number:

144015

Clone Number:

P84

Info:Supplier Page View Product Specs

Browse Similar Products: CD172a Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we were looking for surface molecules that are expressed by PCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

Surface staining with CD172a PE/Dazzle 594 (1/200), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytemeter

Results Summary

PCs express intermediate levels of CD172a.

DOI or PMID #

https://doi.org/10.3389/fimmu.2022.873217

Additional Notes

N/A

Image Gallery

Summary

The Good

It works

The Bad

It is not very bright

The Bottom Line

Can be a good option to stain CD172a.

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