Cell Signaling
PKM2 (D78A4) XP® Rabbit mAb
4053
N/A
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I checked this antibody on fibroblast and I stimulated with TGF-b to overexpress PKM2. Our research is focused on the SMAD pathway that regulates a diverse set of biological processes, including proliferation, differentiation and apoptosis, in many different organ systems. TGF-ß ligands that activate the SMAD-2/3 intracellular pathway have been heavily implicated in fibrosis.
Western Blot
Fibroblast
1:1000, 4 °C, overnight
BSA 5% TBST
1:10000
Chemiluminescence
Data are good and bands are clear.
Band is clear.
Product works very well.