PKM2 (D78A4) XP ® Rabbit mAb 4053S from Cell Signaling Technology

Product Specs
ItemPKM2 (D78A4) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number4053S
Quantity100 µl
ApplicationsW, IP, IHC-P, IF-IC, FC-FP
ReactivityH, M, R, Mk
TargetPKM2
HostRabbit
NCBI Gene AliasesCTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58
ClonalityMonoclonal
NCBI Full Gene Namepyruvate kinase M1/2
Research AreaMetabolism
IsotypeIgG
CloneD78A4
Molecular Weight60

Questions and Answers

Can this antibody be used to study PKM2 in cancer research?

Yes, the PKM2 (D78A4) XP® Rabbit mAb has been utilized in various cancer research studies. For instance, it has been shown to play a role in the HIF-1α/PKM2 axis in glioblastoma cells, which is crucial for tumorigenesis and metabolic reprogramming (). Additionally, it has been implicated in the regulation of metabolic pathways in prostate cancer, enhancing the understanding of PKM2's role in cancer progression ().

Is this antibody effective for immunohistochemistry applications?

The PKM2 antibody has been effectively used in immunohistochemistry, as demonstrated in studies where it was employed to analyze protein expression in various tissue samples (). This application is critical for visualizing PKM2 localization and expression levels in cancer tissues.

What species can this antibody detect?

The PKM2 (D78A4) XP® Rabbit mAb is reactive with multiple species, including human, mouse, rat, and non-human primates. This broad reactivity allows for its application in diverse experimental models across different research areas ().

Are there any specific experimental conditions recommended for using this antibody?

In studies utilizing the PKM2 antibody, it has been noted that proper antigen retrieval methods, such as using EDTA antigen retrieval buffer, are essential for optimal immunofluorescence results (). Additionally, it has been used in Western blotting at a dilution of 1:1000, indicating specific conditions that may enhance its performance in various assays ().

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Bioengineered extracellular vesicles escape lysosomal degradation and deliver Tet-PKM2 for macrophage immunometabolic reprogramming and periodontitis treatmentBioactive MaterialsMay 1, 2026Wen-Jie Zhang, Bei-Min Tian, Fang Li, Xuan Li, Rui-Xin Wu, Yuan Yin, Jia Wang, Dao-Kun Deng, Yu-Zhe Chen, Hong-Yu Wang, et al.anti-CD68 (1:200, Santa Cruz, SC-20060, USA) plus rabbit anti-PKM2 (1:200, Cell Signaling Technology, 4053S, USA), mouse anti-CD68 (1:200, Santa Cruz, SC-20060) plus rabbit anti-iNOS (1:200, Proteintech, 80517-1-RR,

(2) ASO-based PKM splice-switching therapy increases anti-CTLA-4 antibody efficacy in pancreatic ductal adenocarcinomaCell DiscovApril 21, 2026Lijie Han, Lina Gan, Balázs Schäfer, Dillon M. Voss, Mathias Danielsen, Ondrej Kostov, Jia Liu, Ting Wang, Marvin H. Caruthers, Hao Chen, et al.incubated with primary antibodies against PKM2 (1:200; Cell Signaling Technology, rabbit monoclonal, 4053S), PKM1 (1:200; Cell Signaling Technology, rabbit monoclonal, 7067S), CD3ε (1:100; Cell Signaling Technology,

(3) Lysine Acetyltransferase 6A Drives M1 Macrophage Polarization Through Metabolic Reprogramming in Sepsis-Induced Acute Lung InjuryBiomoleculesApril 20, 2026Xin Wang, Junlin Chen, Yimei Lai, Yumeng Wang, Kaixia Hu, Mengshi Wu, Niansheng Yang, Yuefang Huangwith primary antibodies specific for Pyruvate Kinase M2 (PKM2, 1:1000, Cell Signaling Technology, Cat# 4053S), p-PI3Kp85 (1:1000, Cell Signaling Technology, Cat# 17366S), p-AKTS473 (1:2000, Cell Signaling Technology,

(4) Quantitative Phosphoproteomics Identifies Myofibrillar Protein Phosphorylation Mediated by Pyruvate Kinase M2 in BeefFoodsMarch 26, 2026Ying Xu, Xiangfei Liu, Chi Ren, Chengli Hou, Xin Li, Dequan Zhangwere 120 min at 300 mA. The primary antibodies were rabbit antiPKM2 (1:1000 for WB and 1:50 for IP, 4053S, CST, Danvers, MA, USA) and rabbit antimyosin regulatory light chain (1:1000 for WB, ab79935, Abcam,

(5) TPP-Thiazole Derivatives Ameliorate Psoriasiform Inflammation by Glycolysis InhibitionMoleculesMarch 15, 2026Xinwei Meng, Ci-An Cheng, Zhirui Zhang, Siying Qu, Anqi Zhang, Yang Zhang, Jinxin Gu, Hanwen Zhang, Keyue Ding, Lei Fu, et al.4695), STAT3 (CST, 9139), p-STAT3 (Tyr705) (CST, 9145), HK1 (CST, 2024), HSP90 (CST, 4877), PKM2 (CST, 4053), and LDHA (CST, 3582). Images were quantified using ImageJ2 software. 4.7. H&E Staining Following

(6) Regulation of PKM2 expression and function by GLIS3 during metabolic reprogramming in polycystic kidneysExperimental & Molecular MedicineMarch 13, 2026Justin B. Collier, Hong Soon Kang, Sara A. Grimm, Tanushree Mukherjee, Chitrangda Srivastava, Anton M. Jettenantibody at 4 °C with gentle agitation. The primary antibodies used in these studies included PKM2 (4053; Cell Signaling), pPKMS-S37 (11456; SAB), β-catenin (8480; Cell Signaling) and β-actin (MA5-15739; ThermoFisher).

(7) PKM2 phosphorylation by c-SRC activates glycolysis and metastasis with the stimulation of tumor-associated macrophagesCell Communication and SignalingMarch 12, 2026Da Song, Haijie Li, Jialu Xu, Wenli Zhan, Yaqi Chen, Xuelai Luo, Guihua Wang, Xingrui Li, Junbo Hu, Jingqin Lanand Cell Signaling Technology Cat#2101), PKM2 (ABclonal #A20991, and Cell Signaling Technology Cat#4053) , Phospho-Tyrosine (P-Tyr-1000) MultiMab (Cell Signaling Technology Cat#8954), GST-Tag (Cell Signaling Technology

(8) A novel kinase inhibitor, Regorafenib, blocks EGFR-dependent signaling to repress tumour metastasis in human triple-negative breast cancersFront Cell Dev BiolMarch 3, 2026Ludong Zhao, Chunxin Wang, Yanzhi Wang, Meijing Liu, Hongyu Du, Xiaohui Chu, Wenqiang Yang, Wei Zhao, Guan Wang, Bo Zhang, et al.Biological Company (Wuhan, China). p-EGFR (3777S), Trop2 (47866S), p-Src (2105S), p-p38 (4511T), PKM2 (4053T) primary antibody were purchased from Cell Signaling Technology (Beverly, United States). MMP1 (YT2792)

(9) DNA Polymerase Gamma Acetylation Governs Mitochondrial Homeostasis and Vascular Cell SenescenceInternational Journal of Biological SciencesFebruary 4, 2026Pengbo Wang, Liming Yu, Kexin Cao, Xiaofan Guo, Lufan Sun, Shu Zhang, Tong Zhao, Yao Yu, Mengyao Xiong, Chang Liu, et al.(China) Cat #WL02203; p21, Santa Cruz (USA) Cat #sc-6246; PKM2, Cell Signaling Technology (USA) Cat #4053S; PKM2-phosphorylation, Cell Signaling Technology (USA) Cat #3827S; Polγ, Abcam (USA) Cat # ab128899;

(10) NFS1 Regulates IDH2 to Attenuate Abdominal Aortic Aneurysms via Interacting With SP2Advanced ScienceFebruary 4, 2026Luzheng Zhang, Yu Zhang, Dezhong Wen, Suxiang Guo, Xiaohui Qi, Heng Wang, Yujin Sun, Guangdong Yang, Yuehong Wang, Song Xue56298s, CST, Danvers, MA, USA), anti‐ALDOA (1:1000, 8060s, CST, Danvers, MA, USA), anti‐PKM2 (1:1000, 4053s, CST, Danvers, MA, USA), anti‐HA‐Tag (1:1000, 3724t, CST, Danvers, MA, USA), anti‐SP1 (1:1000, 21962‐1‐ap,

(11) CNOT2 /c-Myc/STAT3 signaling is critically involved in glycolysis mediated apoptosis of benzyl isothiocyanate in hepatocellular carcinomaScientific ReportsFebruary 2, 2026Wonil Koh, Su-Yeon Park, Bonglee Kim, Bum-Sang Shim, Sung-Hoon KimCell Signaling Technology, MA, USA), HK2 (#2106, 1:1000; Cell Signaling Technology, MA, USA) , PKM2 (#4053, 1:1000; Cell Signaling Technology, MA, USA), LDH (SC-133123, 1:1000; Santa Cruz, CA, USA) and β-actin

(12) A Melittin-Derived Lead Compound Ameliorates Severe Acute Pancreatitis by Restoring Oxidative Homeostasis and Macrophage MetabolismInflammationJanuary 22, 2026Xiaolong Chen, Ya Chen, Yunyun Mao, Xinxin Chen, Yilin Zhou, Jianfeng Tuovernight with diluted anti-CD206 antibody (1:400; 24595 S, CST, USA) and anti-PKM2 antibody (1:400; 4053 S, CST, USA). Each section was then incubated with proper secondary antibodies. All slides were observed

(13) Metabolic Crosstalk in Triple-Negative Breast Cancer Lung Metastasis: Differential Effects of Vitamin D and E in a Co-Culture SystemCancers (Basel)January 18, 2026Balquees Kanwal, Saranya Pounraj, Rumeza Hanif, Zaklina KovacevicFumarase (Cat.#:4567T), MPC2 (Cat.#:46141S), GLUT1 (Cat.#:73015S), HKII (Cat.#:2867S), PKM2 (Cat.#:4053T), PDH (Cat.#:3205S), LDHA (Cat.#:2012S), MCT1 (Cat.#:76508S), HIF-1α (Cat.#:36169S), and c-Myc (Cat.#:5605S)

(14) Trichostatin A Influences Dendritic Cells’ Functions by Regulating Glucose and Lipid Metabolism via PKM2MoleculesJanuary 16, 2026Xiaoyu Yang, Lihui Men, Yan Guo, Linnan Duan, Meiyi Yu, Leyi Zhang, Tongtong Song, Xiang Li, Xia Chenno. PC0020) was purchased from Beijing Solarbio Science & Technology Co., Ltd. PKM2 (1:1000; cat. no. 4053S) antibody was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA) β-actin (1:1000; cat.

(15) IL-6 regulates the polarization of Dectin-1 macrophages to weaken immune escape in thyroid cancerJournal of Translational MedicineJanuary 10, 2026Xianzhao Deng, Yinyue Wu, Bomin Guo, Zheng Ding, Youben Fan, Jian WangAF7734), anti Rabbit PGK1 (1: 1000, Servicebio, GB11911-100), PKM2 (1:1000, Cell Signaling Technology, 4053T) and anti Rabbit GAPDH (1: 5000, Servicebio, GB15004). After washing, membranes were incubated with

(16) Irisin targets the HK1–glycolysis–NLRP3 pyroptosis axis to prevent chronic kidney disease-associated vascular calcificationRenal FailureJanuary 8, 2026Weijia Xu, Peiwen Wang, Xiaowen Zhu, Zongli Zhang, Xingtong Dong, Ran An, Qi Pang, Aihua ZhangSignaling Technology, Danvers, MA, USA, 2024S), PKM (1:1000; Cell Signaling Technology, Danvers, MA, USA, 4053), anti-NLRP3 (1:500; Abcam, Waltham, MA, USA, ab270449), anti-GSDMD-N (1:500; Cell Signaling Technology,

(17) Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fateCellular and Molecular Life SciencesJanuary 6, 2026Cui Ma, Zhaosi Wang, Xiangrui Zhu, Xiangming Pang, Lixin Zhang, Langlin Ou, Yingli Chen, Yuxiang Liu, Jian Mei, Xiaoyu Guan, et al.(AF7236, 1:500, Beyotime, Shanghai, China), TNF-α (AF8208, 1:500, Beyotime, Shanghai, China), PKM2 (4053, 1:1000, Cell Signaling, MA, US), HK II (66974-1-Ig, 1:1000, Proteintech, IL, USA), PDH (2784, 1:1000,

(18) CD155 reprograms glycolysis via the YAP/TEAD1-GLUT1 axis to promote lung adenocarcinoma progression and M2 macrophage polarization: a metabolic-immune target visualized by 18F-FDG PET/CTJournal of Translational MedicineDecember 22, 2025Zhiming Cheng, Shu Wang, Shuoyan Xu, Yuxiang Wang, Yao Diao, Bulin Du, Xuena Li, Yaming LiAbcam), anti-GLUT1 (1:200, ab115730, Abcam), anti-HK2 (1:200, ab209847, Abcam), anti-PKM2 (1:400, #4053, CST), anti-LDHA (1:300, #3582, CST), anti-CD163 (1:200, ab182422, Abcam), and anti-TGF-β (1:200, ab215715,

(19) lncRNA NKILA Promotes Warburg Effect and Immune Escape in Intrahepatic Cholangiocarcinoma by Regulating the MTX1/TOMM40 AxisMediators InflammDecember 22, 2025Meiying Zhu, Hui Zhu, Zunqiang Zhou, Haiming Zheng, Zhixia Dong, Wenhong Dong, Xinjian WanAbcam, UK; Glut1, ab115730, 46 kDa, 1/100,000, Abcam, UK; LDH, #3582, 32 kDa, 1/1000, CST, USA; PKM2, #4053, 55 kDa, 1/1000, CST, USA; hexokinase2 (HK2), ab209847, 102 kDa, 1/1000, Abcam, UK; monocarboxylate

(20) 2-Deoxyglucose as a therapeutic strategy for EBV-associated gastric carcinoma: Glycolytic and lytic reactivation inhibition under hypoxic conditionsJournal of Virus EradicationDecember 1, 2025Yu Du, Wangsheng Zuo, Yiting Shao, Yanying Ji, Bojin Su, Sihong Liang, Deyu Wang, Bin Li, Yujie Feng, Liping Gong, et al.anti-LDHA antibody (diluted 1:2000; cod. ab52488; Abcam), human anti-PKM2 antibody (diluted 1:1000; cod. 4053T; CST), human anti-HIF-1α antibody (diluted 1:200; cod. ab51608; Abcam), human anti-HIF-2α antibody (diluted

Write a Review

(1)

6/1/2022Great PKM2 Rabbit mAb Antibody from CST Lucy LiuOur research is focused on studying glycolysis pathways of bone marrow derived macrophages. As PKM2 is one of the key enzymes for glycolysis, we checked PKM2 expression by Western Blot. This antibody ... Read Review
(1)

4/28/2022Good PMK2 Antibody for Western Blot Marco PilliI checked this antibody on fibroblast and I stimulated with TGF-b to overexpress PKM2. Our research is focused on the SMAD pathway that regulates a diverse set of biological processes, including ... Read Review
(1)

3/24/2020Great PKM2 Antibody from CST Wei Yang SitHighly recommended antibody for those that wants to detect this protein.
(1)

7/4/2019Excellent PKM2 Antibody for Western from CST Meng SunSince we are interested in looking at tumor metabolism, we looked under PKM2 since this has been an area of interest. With western blot, we looked to see if there was any expression difference when ... Read Review

PKM2 (D78A4) XP ® Rabbit mAb

PKM2 (D78A4) XP ® Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (443)

Reviews

PKM2 (D78A4) XP ® Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map