Western Blot Collagen 1

April 08, 2022

BlueskyReddit
Nanomedicine
Lazar Research Laboratories
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Collagen I antibody

Catalog Number:

ab34710

Clone Number:

N/A

I utilized this antibody for Western blot on fibroblast and I got a lot of unspecific bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Fibroblast

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

Results are not very good.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

N/A

The Bad

A lot of unspecific bands.

The Bottom Line

I used this antibody several times and it works, but with too many unspecific bands.

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