Great CD61 Staining on Platelets

Hem/Onc
Boston Children's Hospital
Postdoctoral Researcher

Overall

Quality of Results

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Company:

BioLegend

Product Name:

PerCP anti-human CD61 Antibody

Catalog Number:

336410

Clone Number:

VI-PL2

Info:Supplier Page View Product Specs

Browse Similar Products: CD61 Antibodies

CD61 (integrin β3) associates with CD41 (integrin αIIb) to form the αIIb/β3 complex, which lays dormant on the surface of resting platelets but undergoes rapid conformational changes following platelet activation, allowing fibrinogen binding and platelet-platelet aggregation. PerCP anti-human CD61 Antibody allows good staining of CD61 on human platelets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood collected in sodium citrate

Primary Incubation

30 min at room temperature

Blocking Agent

Staining buffer contains 0.5% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing PerCP anti-human CD61 Antibody. Lyse/fix buffer was added, and samples were acquired by flow cytometry. PerCP anti-human CD61 Antibody provides good staining of CD61 and allows the clear resolution of platelets from background.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good staining of CD61

The Bad

N/A

The Bottom Line

PerCP anti-human CD61 Antibody provides good staining and allows the clear resolution of platelets from background.

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