PerCP anti-human CD61 336410 from BioLegend

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Frontiers in pharmacology
January 01 2023

Fig 1: Effect of luteolin on collagen- and convulxin-induced platelet aggregation, ATP release, P-selectin expression, and LDH release. Human washed platelets (WPs) were incubated with different concentrations of luteolin or DMSO at 37°C for 10 min after the addition of indicated agonists. (A–D) After incubation, the platelet aggregation and ATP release curves were depicted in real-time, and the inhibition rate curves were drawn (A, far right) to calculate the IC50 values using nonlinear regression. (B) Platelet aggregation curves under different stimulations. (C) The platelet aggregation rates were calculated from the curves in (B). (D) ATP release and inhibition rate IC50 values using nonlinear regression (E) Flow cytometric scatter plots were performed for CD62p and CD61. (F) Platelets were incubated at 37°C for 60 min with different doses of luteolin, DMSO, or Triton X-100. The LDH release was measured. All experimental results are expressed as mean ± SEM (n ≥ 3). Compared with the Vehicle(DMSO) group, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data were analyzed with one-way ANOVA followed by Dunnett’s test.
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Scand J Immunol
July 28 2025

Fig 2: In‐depth analysis of platelet–neutrophil complex (PNC) formation after stimulation with PAF in diluted whole blood in vitro. (A) Representative light microscopy of neutrophil not in complex with platelets (PMN, PBS++‐stimulated Ctrl) and an exemplary PNC (PAF‐stimulated) and (B) summary data for the percentage of PNCs detected by light microscopy and manual counting. (C) Representative flow cytometry gating using CD61 median fluorescence intensity (MFI) for PNCs after stimulation with PBS++ as control (Ctrl) or PAF and (D) summary data for the percentage of PNCs detected by flow cytometry. (E) Concentration and (F) time dependency of PNC formation after PAF stimulation. For the concentration dependency, neutrophils were stimulated with PAF concentrations from 10 μM to 100 pM. The value 0% corresponds to neutrophils stimulated with PBS++ as control (Ctrl) and 100% represents the maximum effect of neutrophils stimulated with PAF. For all other analyses the used PAF concentration was 1 μM. n = 5–10, median ± interquartile range. **p < 0.01, ***p < 0.001, and ****p < 0.0001, respectively. Mann–Whitney U‐test for (B, D, F) neutrophils stimulated with PBS++ as control (Ctrl) versus neutrophils stimulated with PAF and in (F) for each time point (15, 30, 60 and 120 min).

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PerCP anti-human CD61

BioLegend's PerCP anti-human CD61 is a Mouse monoclonal antibody. The PerCP anti-human CD61 Antibody was generated using ITGB3 as the antigen. It reacts with Homo Sapiens, Human, and Non-Human Primate. This antibody has been shown to work in applications such as: Flow Cytometry.

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PerCP anti-human CD61 [VI-PL2]

Frontiers in pharmacology

Scand J Immunol

PerCP anti-human CD61 from BioLegend

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BioLegend

Citations (4)

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PerCP anti-human CD61 from BioLegend

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