Good CD107a Staining on Human Platelets

April 05, 2022

BlueskyReddit
Hem/Onc
Boston Children's Hospital
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

PE-Cy5 Mouse Anti-Human CD107a

Catalog Number:

555802

Clone Number:

H4A3

Info:Supplier Page View Product Specs

Browse Similar Products: CD107a Antibodies

CD107a is a lysosomal marker that is expressed on the surface of activated/degranulated platelets. PE-Cy5 Mouse Anti-Human CD107a allows good staining of CD107a on human platelets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood collected in sodium citrate

Primary Incubation

30 min at room temperature

Blocking Agent

Staining buffer contains 0.5% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing PE-Cy5 Mouse Anti-Human CD107a with or without 10 µg/mL CRP-XL (platelet agonist). Lyse/fix buffer was added, and samples were acquired by flow cytometry. This antibody allowed good staining of CD107a on agonist-stimulated platelets.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good staining of CD107a

The Bad

N/A

The Bottom Line

PE-Cy5 Mouse Anti-Human CD107a allows good staining of CD107a on the surface of human blood platelets.

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