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PE/Cyanine7 anti-human CD63 Antibody
353010
H5C6
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CD63 is present in the granules and/or lysosomes of resting platelets and expressed on the cell surface following platelet activation. PE/Cyanine7 anti-human CD63 Antibody allows the clear resolution of activated/degranulated platelets from resting/nondegranulated platelets.
Flow Cytometry
Human whole blood collected in sodium citrate
30 min at room temperature
Staining buffer contains 0.5% BSA
N/A
Fluorescence
Citrate-anticoagulated human whole blood was diluted 1:10 in HEPES-Tyrodes-BSA containing PE/Cyanine7 anti-human CD63 Antibody with or without 10 µg/mL CRP-XL (platelet agonist). Lyse/fix buffer was added, and samples were acquired by flow cytometry. PE/Cyanine7 anti-human CD63 Antibody allowed clear resolution of activated/degranulated platelets.
Bright signal allows good resolution of activated/degranulated platelets.
This antibody allows good detection of CD63 on the surface of activated human platelets.