Time Course Effect of Chemoattractant on IL-33 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

R&D Systems

Product Name:

Mouse IL-33 Antibody

Catalog Number:

AF3626

Clone Number:

N/A

Info:Supplier Page View Product Specs

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IL-33 is a proinflammatory protein that may also regulate gene transcription. We checked the time course effect of IL-33 expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

IL-33 was identified based on sequence and structural homology with IL-1 family cytokines. Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment. Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins. We found time dependent increase of Il-33 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Antibody works great for western blot.

The Bad

None

The Bottom Line

Highly recommended antibody for western blot.

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