IL-33 Antibody AF3626 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-33 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF3626
NCBI Gene Aliases9230117N10Rik, NF-HEV, C9orf26, IL1F11, Il-33, NFEHEV, DVS27, Il1f11
ApplicationsELISA Development,ELISA Development (Capture),ELISpot Development,Neutralization Control,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Neutralization,ELISA Capture (Matched Antibody Pair),Immunofluorescence,Intracellular Staining by Flow Cytometry,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,CyTOF-ready
ReactivityHuman,Mouse,Rat,Transgenic Mouse
HostGoat
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
TargetIL-33
NCBI Full Gene Nameinterleukin 33
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse IL-33, Ser109-Ile266, Accession # Q8BVZ5
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse IL-33 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human IL-33 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study IL-33 in mouse models, and if so, how has it been applied in research?

Yes, the IL-33 antibody has been effectively used in mouse models. For instance, it was utilized in a study examining the role of IL-33 in osteoclastogenesis, where it was shown that IL-33 expression in osteoblasts inhibited osteoclast differentiation through the microRNA-34a-Notch1 pathway (). Additionally, it has been used in immunofluorescence assays to detect IL-33 in skin samples from mice with atopic dermatitis, highlighting its relevance in inflammatory skin disorders ().

What is the specificity of this antibody when detecting IL-33 in different species?

The IL-33 antibody is specific for mouse IL-33, with less than 2% cross-reactivity observed with recombinant human IL-33 in sandwich immunoassays. This specificity makes it suitable for studies focused on mouse models while minimizing interference from human IL-33 ().

How has this antibody been utilized in the context of immune response studies?

The IL-33 antibody has been employed in various studies to investigate its role in immune responses. For example, it was used to demonstrate that IL-33 levels are suppressed in the lungs of mice exposed to secondhand smoke, which correlated with impaired immune responses and bacterial clearance in a model of muco-obstructive lung disease (). This underscores the antibody's utility in exploring the immunological implications of IL-33.

Are there any notable applications of this antibody in studying pathological conditions?

Yes, the IL-33 antibody has been applied in research on pathological conditions such as endometriosis. It was shown that IL-33 contributes to the expansion of group 2 innate lymphoid cells, which modulates the inflammatory microenvironment in endometriotic lesions. Neutralization of IL-33 using this antibody was suggested as a potential therapeutic strategy for alleviating symptoms associated with endometriosis ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Gegen Qinlian Decoction Mitigates DSS-Induced Acute Colitis and Reinstates Gut Barrier Function in Mice, Correlating with Suppressed IL-33/ST2 SignalingJ Inflamm ResJuly 10, 2026Yuqi Wu, Mengqing Ma, Liuru Luo, Weijian Zhang, Xinxin Hong, Xiaoyan Jiang, Junyi Li, Sihui Zeng, Kexin Li, Huishan Yuan, et al.Cat. No. 580508) was obtained from BioLegend (San Diego, CA, USA). The Mouse IL-33 Antibody (Cat. No. AF3626) was procured from R&D Systems (Minnesota, USA). Gegen Qinlian Decoction (GGQLD) was prepared by the

(2) Critical role of keratinocytes and protease-activated receptor 2 in secondary lymphedema developmentClin Transl MedJune 1, 2026Hyeung Ju Park, Sarit Pal, Xizhao Chen, Jinyeon Shin, Gabriela D. García Nores, Jung Eun Baik, Annica Stull-Lane, Abraham J. Book, Cristina C. Clement, Elizabeth M. Encarnacion, et al.,Guinea pig,OriGene,BP5009 Ki67,Rat,Invitrogen,14‐5689‐82 IL‐1α,Rabbit,Abcam,ab9614 TSLP,Rabbit,Abcam,ab188766 IL‐33,Goat,R&D Systems,AF3626 PAR2,Rabbit,Abcam,ab180953 KLK5,Rat,R&D Systems,MAB7236 NFATc1,Rabbit,Thermo Fisher Scientific,PA5‐90432 EGF,Rabbit,Abcam,ab9695 EGFR,Rabbit,Abcam,ab52894 Lyvel,Rabbit,Abcam,ab14917 CD3e,Hamster,Thermo Fisher Scientific

(3) Influenza A/PR8 virus infection in mice suppresses basal and Oncostatin M-induced IL-33 expression in vivo and in vitroScientific ReportsMay 23, 2026Leila Somani-Davis, Anisha Dubey, Fernando Botelho, Lily Buder, Kyle MacDonald, Matthew Miller, Carl D. Richards(TBS/Odyssey Blocking buffer – Mandal, Guelph, Canada), primary antibodies mouse IL-33 (R&D Systems Cat#AF3626), mouse Arg-1 (BD Biosciences Cat#610708), pSTAT3 (Cell Signaling Cat#9145), STAT3 (Cell Signaling Cat#4901),

(4) The alarmin interleukin-33 modulates platelet proteome, function, and biogenesisBlood AdvancesApril 14, 2026Lucie Gelon, Stéphane Roga, Anne Gonzalez-de-Peredo, Edith Vidal, Jean-Philippe Girard, Sonia Severin, Emma Lefrançaisdiscrepancies, we performed analyses with a polyclonal goat anti-mouse IL-33 antibody (R&D Systems, catalog no. AF3626) that has been validated for immunofluorescence, flow cytometry, and western blot analyses in previous

(5) DDR1 Promotes Immune Evasion in Colorectal Cancer by Orchestrating IL33-Mediated M2-like Polarization of Tumor-Associated MacrophagesCancer Immunol ResApril 10, 2026Xiaofan Duan, Gaoshaer Yeerkenbieke, Yanjun Feng, Mingwang Zhou, Yumei Zhang, Jiuli Zhou, Jin Liconditioned media from Ddr1-OE MC38 cells in the presence of 0.2 μg/mL anti-IL33 neutralizing antibody (#AF3626, R&D Systems) or isotype Ctrl (#AB-108-C, R&D Systems) for 48 hours. For IL33 stimulation assays, BMDMs

(6) Lung adventitial fibroblasts support type 2 Tregs to shape the response to influenza infectionbioRxivApril 9, 2026Anthony A. Chang, Joanna Balcerek, Sofia E. Caryotakis, Kennedi B. Pyper, Peri Matatia, Elina B. Wells, Tatsuya Tsukui, Nathan A. Ewing-Crystal, Eric Dean Merrill, Nick M. Mroz, et al.anti-Pi16 (R&D Systems AF4929), rabbit anti-proSP-C (Millipore AB3786), goat anti-IL-33 (R&D Systems AF3626), and rat anti-EpCAM (BD Pharmingen 552370). Directly conjugated antibodies were also used including

(7) Single-cell multiomics uncovers an endothelial mechanosensitive PIEZO1-IL-33 axis driving pulmonary fibrosisNature communicationsMarch 20, 2026Zhang L, Gui X, Hou R, Jia L, Xia S, Zhang X, Fu Y, Meng QF, Luo Q, Shi X, et al.CD31 (Abcam, #ab9498; Servicebio, #GB13063), VE-cadherin (R&D Systems, #AF938), IL-33 (R&D Systems, #AF3626), PIEZO1(proteintech, #15939-1-AP) and αSMA (Abcam, #ab7817) antibodies. Cytokine measurement by ELISA The

(8) Spatial Transcriptomics of TMJ Reveals a Remodeling Fibroblast‐Immune Microenvironment Driving Arthritis PainAdvanced ScienceMarch 1, 2026Ziying Lin, Supawadee Jariyasakulroj, Yang Shu, Jingyi Chen, Qing Chang, Pao‐Fen Ko, Yuyueyang Qiu, Feixiang Chen, David Ahn, Zhen Zhao, et al.Rabbit anti‐PDGFRα (Cell Signaling Technology, Cat #5241), Goat anti‐IL‐33 (R&D system, AF3625 and AF3626), Rabbit anti‐CD206 (Abcam, ab64693), Rabbit anti‐TUBB3 (Cell Signaling Technology, Cat #5666S), and

(9) Fibroblast-derived alarmin promotes oral wound healing by activating regulatory T cells that relay pro-angiogenic and anti-inflammatory responsesCell reportsJanuary 27, 2026Chen Z, Huang Z, Kim WS, Debnath R, Prasongyuenyong K, DerGarabedian BP, Do T, Moore-Kosslow MA, Korostoff JM, Chen C, et al.KRT14,Biolegend,Cat. #: 906004;RRID: AB_2616962 Goat polyclonal anti-mouse IL33,R&D Systems,Cat. #: AF3626;RRID: AB_884269 Goat polyclonal anti-mouse COL3A1,Novus Biologicals,Cat. #: NBP1-26547;RRID: AB_1853195 Rat

(10) Immunomodulatory Functions of Intercalated Cells in Kidney AutoimmunitybioRxivJanuary 16, 2026Maria C. Avenatti, Maia L. Elizagaray, Micah C. Purba, Ferran Barrachina, Angela Chen, Isinsu Bastepe, Katherine Radovanovic, Maria A. Battistonegoat anti-mouse-CD2034 (0.5mg/mL, sc-7735, Santa Cruz), goat anti-mouse IL-33 (0.2 mg/mL; Polyclonal; AF3626, R&D Systems), rat anti-mouse CD138 (0.2 mg/ml; Clone 281–2, 563192, BD Biosciences) and Alexa Fluor

(11) ERα activates NAMPT/IL-33 signaling to enhance beige thermogenesis and metabolic fitnessScience AdvancesJanuary 9, 2026Ruoci Hu, Jooman Park, Yanyu Qian, Shaolei Xiong, Ahmad Hamza Elsabbagh, Aditya Chhikara, Huailing Fan, Zuoxiao Shi, Lifeng Liu, Yanhui Li, et al.of mouse IgG control (1 μg/mouse; Invitrogen 31903) or anti-IL-33 antibody (1 μg/mouse; R&D Systems AF3626) by intraperitoneal injection for five consecutive days. Metabolic phenotyping experiments Mice were

(12) Electroacupuncture ameliorates incisional pain via suppressing IL-33 signaling-related macrophage infiltration and ROS overproduction in incised skinChinese MedicineJanuary 9, 2026Ruoyao Xu, Kaige Zheng, Yushuang Pan, Peiyi Li, Muyan Chen, Huimin Nie, Boyu Liu, Yan Tai, Xiaofen He, Junying Du, et al.at room temperature, and then the membranes were incubated with primary antibodies: IL-33 (1:500, #AF3626, R&D Systems, USA) overnight at 4 °C. Subsequently, the immunoblots were incubated with the secondary

(13) IL-33 blockade attenuates vascular inflammation in a mouse model of Kawasaki disease vasculitisClinical and Experimental ImmunologyJanuary 6, 2026Thacyana T. Carvalho, Benjamin L. Ross, Prasant K. Jena, Asli E. Atici, Angela C. Gomez, Michael C. Fishbein, Emily A. Aubuchon, Youngho Lee, Richard T. Lee, Elizabeth A. Jacobsen, et al.IL-33 neutralization was performed using an anti-IL-33 antibody (Mouse IL-33 Antibody; R&D Systems; AF3626). An isotype control IgG (IgG from goat serum; Sigma-Aldrich, I5256) was used as a control. Antibodies

(14) Complement-producing adventitial fibroblasts form an IL-33 alarmin hub that maintains ILC2s during airway allergyCell reportsDecember 23, 2025Atakkatan A, Magesh A, Yong HM, Murray E, Gour N, Lajoie S.

(15) IL33-induced lipid droplet formation in mature low-density neutrophils drives colorectal cancer liver metastasisCellular & Molecular ImmunologyNovember 10, 2025Yuchen Zhang, Suyue Yu, Dina Yeernuer, Wangyi Liu, Zhuoqing Xu, Wenqing Feng, Zeping Lv, Xuanhao Liu, Peiqi Tan, Minhua Zheng, et al.with 3.6 μg/mouse recombinant murine IL33 (R&D Systems; PBS control), 5 μg/mouse anti-IL33 antibody (AF3626, R&D Systems; AB-108-C, R&D Systems), or 200 μg/mouse anti-PD-L1 antibody (CP001, BioXCell; IgG control,

(16) Sexual dimorphism in colon is mediated by an androgen-IL33+ stromal cell axisCell BiosciOctober 29, 2025Haoyu Wang, Baowei Jing, Juan Zou, Tian Lan, Mengxin Hu, Lan Lin, Hanhua Cheng, Rongjia ZhouAnti-AR (A19611, ABclonal), Anti-HSD17B2 (A1983, ABclonal), Anti-HSD3B2/3 (A1823, ABclonal), Anti-IL-33 (AF3626, R&D systems), Anti-SerpinA3N (AF4709, R&D systems), Anti-GAPDH (60004-1-Ig, Proteintech), Anti-Ki67

(17) Choroid Plexus Fibroblast–ILC2 Niche Promotes Adult Hippocampal Neurogenesis after Traumatic Brain InjuryAdvanced ScienceSeptember 1, 2025Shiqi Gao, Xiaoming Guo, Sixuan Tian, Huaping Huang, Caidi Ying, Junjie Wang, Jiahao Zhang, Jun Lin, Anwen Shao, Jingyu Wang, et al.buffer (P0013, Beyotime) and sonicate for 25 s, and the antibody used was Goat anti‐IL33 antibody (AF3626, R&D Systems). Primary Murine NSCs Culture Primary NSCs were obtained from adult (5–6 weeks) mice

(18) Polystyrene particles induces asthma-like Th2-mediated lung injury through IL-33 secretionEnvironment internationalSeptember 1, 2025Woo JH, Song MK, Baek SH, Park CJ, Kim JW, Kim B, Lee K.

(19) Oligodendrocyte-derived IL-33 regulates self-reactive CD8+ T cells in CNS autoimmunityJ Exp MedJuly 7, 2025Nicolas Fonta, Nicolas Page, Bogna Klimek, Margot Piccinno, Giovanni Di Liberto, Sylvain Lemeille, Mario Kreutzfeldt, Anna Lena Kastner, Yusuf I. Ertuna, Ilena Vincenti, et al.(PBS/1% BSA). After antigen retrieval, sections were incubated with primary antibody for IL-33 (Cat# AF3626; R&D Systems) and Olig2 (Cat# 18953; R&D Systems). Bound primary antibodies were visualized with anti-goat

(20) Site- and cell-type-specific miRNA and mRNA genes and networks across the cortex, striatum, and hypothalamusCommun BiolJuly 1, 2025Amanda M. Zacharias, Ciara D. O'Connor, Danai G. Topouza, Zhi Yi Fang, Helia Ghazinejad, Hanlin Chen, Qingling Duan, Nader Ghasemlouwith primary antibodies. Primary antibodies for proteins of interest were anti-IL-33 (1:50, cat.no.AF3626, R&D Systems), anti-AdipoR2 (1:200, cat.no. BS-0611R, Bioss), anti-FZD4 (1:200, cat.no. SAB4503265,

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3/31/2022Time Course Effect of Chemoattractant on IL-33 Expression Somasundaram RaghavanIL-33 is a proinflammatory protein that may also regulate gene transcription. We checked the time course effect of IL-33 expression by chemoattractant.

Mouse IL-33 Antibody

IL-33 Antibody

IL-33 Antibody

IL-33 Antibody

IL-33 Antibody

IL-33 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (186)

Reviews

IL-33 Antibody from R&D Systems, a Bio-Techne Brand

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