Good Effector T Cell Antibody

March 09, 2022

BlueskyReddit
Chemical/Biomedical Engineering
University of Toronto
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD

Product Name:

aCD8a BUV805

Catalog Number:

612898

Clone Number:

53-6.7

Info:Supplier Page View Product Specs

Browse Similar Products: CD8a Antibodies

We study the mechanisms responsible for establishing immune tolerance using single cell analysis methods including flow cytometry, single cell omics, etc.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse LN and thymus

Primary Incubation

Block: 1:50 in 100uL at 37C for 30 min

Blocking Agent

Fc Block (aCD16/32)

Secondary Incubation

L/D: Zombie NIR 1:4000 in 100 uL at RT for 20 min

Tertiary Incubation

Surface stain: 1:200 in 100uL at 4C for 30 min

Detection

Spectral flow cytometry (Cytek aurora)

Results Summary

This antibody works great for identifying effector T cells after first gating on CD3e+ cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very affordable, stains well

The Bad

Have to spend some time to optimize staining concentration to get good performance for spectral flow.

The Bottom Line

A great effector T cell antibody.

Share your experience with other scientists. Write a Review! »

Join the discussion