Good For Spectral FC

Chemical/Biomedical Engineering
University of Toronto
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD4 BV711

Catalog Number:

100557

Clone Number:

RM4-5

Info:Supplier Page View Product Specs

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We study the mechanisms responsible for establishing immune tolerance using cutting edge single cell analysis methods like spectral flow cytometry, single cell omics, etc.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse thymus and lymph nodes

Primary Incubation

Block: 1:50 in 100uL at 37C for 30 min

Blocking Agent

Fc Block (aCD16/32)

Secondary Incubation

L/D: Zombie NIR 1:4000 in 100 uL at RT for 20 min

Tertiary Incubation

Surface stain: 1:100 in 200uL at 4C for 30 min

Detection

Spectral flow cytometry (Cytek aurora)

Results Summary

This antibody is great for identifying CD4+ helper T cells. Works well both in a dump channel to help remove T cells to purify myeloid lineages and as a primary marker to subset T cells further into regulatory and memory CD4 T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

A great T cell antibody at a reasonable price.

The Bad

Somewhat high concentration required for staining.

The Bottom Line

Perfect for flow cytometry analysis of commonly studied immune populations.

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