Good SOX3 Antibody for Immunostaining

March 01, 2022

Department of Pediatrics
Children's mercy hospital
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human SOX3 Antibody

Catalog Number:

AF2569

Clone Number:

N/A

Info:Supplier Page View Product Specs

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Constructed mice tissue immunostained for sox3. Signal works well, expression increased compared to WT control mice.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse heart

Primary Incubation

1:250 dilution overnight incubation

Blocking Agent

1% BSA prepared in 1X pbs

Secondary Incubation

1:250 dilution 1% BSA prepared in 1X pbs for 1 hr

Tertiary Incubation

None

Detection

Cofocal

Results Summary

In our experimental aortic constructed mice heart were immunostained for sox3; expression is very high compared to WT control mice.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works great for single stain compensation

The Bad

N/A

The Bottom Line

Signal is very good and highly recommended this antibody for immunostaining experiments.

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