VCAM-1 Staining for Fluorescence Imaging

February 25, 2022

BlueskyReddit
Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

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Company:

ThermoFisher

Product Name:

VCAM-1 monoclonal antibody

Catalog Number:

MA5-11447

Clone Number:

1.4C3

VCAM-1 monoclonal antibody was used to characterize the response of primary mouse brain endothelial cells to inflammatory cytokine TNFa.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Primary mouse brain endothelial cells

Primary Incubation

1:500 in PBS+0.1% Tween-20 overnight incubation at 4C

Blocking Agent

PBS+0.1% Tween-20 + 10% BSA

Secondary Incubation

Goat anti-rat IgG AF594 1:500 in PBS+0.1% Tween-20 2hr incubation at 25C

Tertiary Incubation

N/A

Detection

Microscopy

Results Summary

VCAM-1 stained brightly and cleanly, allowing for analysis by microscopy.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Highly specific staining at high dilution factor

The Bad

Some background aggregation

The Bottom Line

Bright VCAM-1 staining of fixed endothelial cells at 1:500 dilution.

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